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DOE OSTI · 2519695

RWRtoolkit Narrative

Kainer, David·Lane, Matthew·Sullivan, Kyle·Miller, J. Izaak·Cashman, Mikaela·Morgan, Mallory·Cliff, Ashley·Romero, Jonathon·Walker, Angelica·Blair, D. Dakota·Chhetri, Hari·Wang, Yongqin·Pavicic, Mirko·Furches, Anna·Noshay, Jaclyn·Drake, Meghan·Landry, Natalie·Ireland, AJ·Missaoui, Ali·Kang, Yun·Sedbrook, John·Dehal, Paramvir·Canon, Shane·Jacobson, Daniel
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Abstract

This narrative presents a demonstration of RWRtools methods to the important bioenergy feedstock Panicum virgatum (Switchgrass) by leveraging networks from the model plant Arabidopsis thaliana. We used a genome-wide association study (GWAS) to identify single nucleotide polymorphisms (SNPs) associated with Switchgrass shoot biomass. We then assigned these SNPs to Switchgrass genes, and using Phytozome (Goodstein et al., 2012), assigned these 38 Switchgrass GWAS genes to 32 Arabidopsis orthologs. These 32 genes are the starting set of genes (i.e. the seed gene set) for this demo narrative.

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Kainer, David, Lane, Matthew, Sullivan, Kyle, Miller, J. Izaak, Cashman, Mikaela, Morgan, Mallory, Cliff, Ashley, Romero, Jonathon, Walker, Angelica, Blair, D. Dakota, Chhetri, Hari, Wang, Yongqin, Pavicic, Mirko, Furches, Anna, Noshay, Jaclyn, Drake, Meghan, Landry, Natalie, Ireland, AJ, Missaoui, Ali, Kang, Yun, Sedbrook, John, Dehal, Paramvir, Canon, Shane, Jacobson, Daniel. 2024-07-16. RWRtoolkit Narrative. https://doi.org/10.25982/165213.26%2F2519695

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