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DOE OSTI · 2477669

High Throughput Genome Releaser

Yuan, Guoliang·Salalila, Aljon L.·Hwang, SungJoo·Deng, Zhiqun·Deng, Shuang
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Abstract

In this study, we present the development of a High Throughput Genome Releaser, an innovative device addressing common challenges in screening PCR. This genome DNA releaser is designed for rapid, cost-effective, and efficient DNA extraction, optimized for subsequent PCR reactions. Our experimentation with various synthetic materials led us to select a particular type of plastic that mirrors the properties of glass cover slides, providing a smooth surface and effective compression capabilities. We engineered a 96-well device equipped with a 96-well plate and a top rod, operable both manually and automatically, which is compatible with widely used liquid-handling robot decks. This compatibility enhances ease of use in high-throughput PCR setups. Additionally, we developed software to support its automatic functions. The genome releaser facilitates the extraction of PCR-amplifiable genomic DNA from 96 samples within minutes, eliminates the need for extraction buffers, and is adaptable to a wide range of microorganisms and cells. This versatility could significantly advance biomanufacturing processes.

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42 ENGINEERING59 BASIC BIOLOGICAL SCIENCESDeoxyribonucleic AcidGenomeGraphical User Interface

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Yuan, Guoliang, Salalila, Aljon L., Hwang, SungJoo, Deng, Zhiqun, Deng, Shuang. 2024-09-30. High Throughput Genome Releaser. https://doi.org/10.2172/2477669

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