DOE OSTI · 1892659
Methods of making guide RNA
Abstract
A nucleic acid construct is provided that encodes two or more or a plurality of spacer sequences separated by restriction endonuclease recognition site. A plurality of such nucleic acid sequences are provided as a library for making guide RNAs for use with CRISPR/Cas systems.
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Chavez, Alejandro, Hu, Johnny Hao. 2022-03-01. Methods of making guide RNA. https://www.osti.gov/biblio/1892659
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