Chapter 7 - CRISPR-Based Tools for Microbial Cell Factories

Liu, Rongming; Liang, Liya; Stettner, Sean; Freed, Emily F.; Eckert, Carrie A. (ORCID:0000000342012926)

doi:10.1016/b978-0-12-821477-0.00001-5

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Chapter 7 - CRISPR-Based Tools for Microbial Cell Factories

Liu, Rongming·Liang, Liya·Stettner, Sean·Freed, Emily F.·Eckert, Carrie A. (ORCID:0000000342012926)

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Abstract

The development of microbial chassis for the production of a variety of biochemicals and biofuels is a growing area of research. How to efficiently manipulate genetic information to achieve optimal production of these compounds is a key area of focus in the field. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become a popular strategy for gene editing and regulation in many organisms due to its versatility and efficacy. Here, we describe methods developed utilizing CRISPR-Cas systems for engineering microbial cell factories (e.g., bacteria and yeast) at the single gene to genome scale for mutagenesis and transcriptional regulation of target genes. Finally, we provide a perspective on the challenges and opportunities for the applications of advanced CRISPR-Cas-based tools for engineering microbial cell factories.

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BIOMASS FUELS CRIPSRa CRISPR-Cas CRISPRi gene editing genome editing

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Liu, Rongming, Liang, Liya, Stettner, Sean, Freed, Emily F., Eckert, Carrie A. (ORCID:0000000342012926). 2021-03-08. Chapter 7 - CRISPR-Based Tools for Microbial Cell Factories. https://doi.org/10.1016/b978-0-12-821477-0.00001-5

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