Synthetic methylotrophs

Papoutsakis, Eleftherios T.; Whitaker, William Brian; Bennett, Robert Kyle

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DOE OSTI · 1650848

Synthetic methylotrophs

Papoutsakis, Eleftherios T.·Whitaker, William Brian·Bennett, Robert Kyle

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Abstract

The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.

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09 BIOMASS FUELS 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY 59 BASIC BIOLOGICAL SCIENCES

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Papoutsakis, Eleftherios T., Whitaker, William Brian, Bennett, Robert Kyle. 2020-05-05. Synthetic methylotrophs. https://www.osti.gov/biblio/1650848

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