Quantitative substrate utilization in microbial ecology using stable isotope probing

Hungate, Bruce; Schwartz, Egbert; Mau, Rebecca; Marks, Jane

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DOE OSTI · 1600267

Quantitative substrate utilization in microbial ecology using stable isotope probing

Hungate, Bruce·Schwartz, Egbert·Mau, Rebecca·Marks, Jane

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Abstract

Methods modifying conventional SIP so that isotopic incorporation into the genomes of individual microbial taxa can be quantified are described. Further, methods to quantify the baseline densities of the DNA of individual microbial taxa without exposure to isotope tracers and then to quantify the change in DNA density of each taxon caused by isotope incorporation are described. The distribution of DNA of each taxon along a density gradient reflects the influence of isotope incorporation only, without reflecting the guanine-plus-cytosine content.

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Hungate, Bruce, Schwartz, Egbert, Mau, Rebecca, Marks, Jane. 2019-11-12. Quantitative substrate utilization in microbial ecology using stable isotope probing. https://www.osti.gov/biblio/1600267

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