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DOE OSTI · 1151768

Chip-based sequencing nucleic acids

Beer, Neil Reginald
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Abstract

A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

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BibTeXRIS

Beer, Neil Reginald. 2014-08-26. Chip-based sequencing nucleic acids. https://www.osti.gov/biblio/1151768

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