NASA NTRS · 20030005598
Two-Photon Fluorescence Correlation Spectroscopy
Abstract
We will describe a two-photon microscope currently under development at the NASA Glenn Research Center. It is composed of a Coherent Mira 900 tunable, pulsed Titanium:Sapphire laser system, an Olympus Fluoview 300 confocal scanning head, and a Leica DM IRE inverted microscope. It will be used in conjunction with a technique known as fluorescence correlation spectroscopy (FCS) to study intracellular protein dynamics. We will briefly explain the advantages of the two-photon system over a conventional confocal microscope, and provide some preliminary experimental results.
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Zimmerli, Gregory A., Fischer, David G.. 2002-11-01. Two-Photon Fluorescence Correlation Spectroscopy. https://ntrs.nasa.gov/citations/20030005598
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