NASA NTRS · 19950018111
Fusion proteins as alternate crystallization paths to difficult structure problems
Abstract
The three-dimensional structure of a peptide fusion product with glutathione transferase from Schistosoma japonicum (SjGST) has been solved by crystallographic methods to 2.5 A resolution. Peptides or proteins can be fused to SjGST and expressed in a plasmid for rapid synthesis in Escherichia coli. Fusion proteins created by this commercial method can be purified rapidly by chromatography on immobilized glutathione. The potential utility of using SjGST fusion proteins as alternate paths to the crystallization and structure determination of proteins is demonstrated.
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Carter, Daniel C., Rueker, Florian, Ho, Joseph X., Lim, Kap, Keeling, Kim, Gilliland, Gary, Ji, Xinhua. 1994-01-01. Fusion proteins as alternate crystallization paths to difficult structure problems. https://ntrs.nasa.gov/citations/19950018111
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