Functional colloidal particles for immunoresearch

Abstract

The paper deals with the development of a new class of immunological reagents consisting of antibodies covalently bonded to polymeric microspheres to serve as convenient markers for detection of cell surface antigens by scanning electron and light microscopy. Attention is focused on the design and synthesis of spherical particles containing hydroxyl and carboxyl groups on their surface in a wide range of sizes (30-340 nm diam) by emulsion copolymerization, the preparation of spherical particles ranging from 300 nm to 3 microns and containing a variety of functional groups by means of ionizing radiation, and the experimental conditions for the covalent bonding of fluorescent molecules and antibodies to the spheres by means of the cyanogen bromide, carbodiimide and glutaraldehyde methods. These reagents are used to locate antigens on red blood cells, on mouse and human lymphocytes, and on the surface of photoreceptors. They offer a number of advantages and applications for the study of cell surfaces for immunodiagnosis.