Engineering topics

Xu, Q.

Publications and source records attributed to Xu, Q..

7 records

NASA NTRS1995

Mutational analysis of photosystem I polypeptides in the cyanobacterium Synechocystis sp. PCC 6803. Targeted inactivation of psaI reveals the function of psaI in the structural organization of psaL

We cloned, characterized, and inactivated the psaI gene encoding a 4-kDa hydrophobic subunit of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803. The psaI gene is located 90 base pairs downstream from psaL, and is transcribed on 0.94- and 0.32-kilobase transcripts. To identify the function of PsaI, we generated a cyanobacterial strain in which psaI has been interrupted by a gene for chloramphenicol resistance. The wild-type and the mutant cells showed comparable rates of photoautotrophic growth at 25 degrees C. However, the mutant cells grew slower and contained less chlorophyll than the wild-type cells, when grown at 40 degrees C. The PsaI-less membranes from cells grown at either temperature showed a small decrease in NADP+ photoreduction rate when compared to the wild-type membranes. Inactivation of psaI led to an 80% decrease in the PsaL level in the photosynthetic membranes and to a complete loss of PsaL in the purified photosystem I preparations, but had little effect on the accumulation of other photosystem I subunits. Upon solubilization with nonionic detergents, photosystem I trimers could be obtained from the wild-type, but not from the PsaI-less membranes. The PsaI-less photosystem I monomers did not contain detectable levels of PsaL. Therefore, a structural interaction between PsaL and PsaI may stabilize the association of PsaL with the photosystem I core. PsaL in the wild-type and PsaI-less membranes showed equal resistance to removal by chaotropic agents. However, PsaL in the PsaI-less strain exhibited an increased susceptibility to proteolysis. From these data, we conclude that PsaI has a crucial role in aiding normal structural organization of PsaL within the photosystem I complex and the absence of PsaI alters PsaL organization, leading to a small, but physiologically significant, defect in photosystem I function.

NASA Discipline Cell Biology↗

NASA NTRS1994

Identification of surface-exposed domains on the reducing side of photosystem I

Photosystem I (PSI) is a multisubunit enzyme that catalyzes the light-driven oxidation of plastocyanin or cytochrome c6 and the concomitant photoreduction of ferredoxin or flavodoxin. To identify the surface-exposed domains in PSI of the cyanobacterium Synechocystis sp. PCC 6803, we mapped the regions in PsaE, PsaD, and PsaF that are accessible to proteases and N-hydroxysuccinimidobiotin (NHS-biotin). Upon exposure of PSI complexes to a low concentration of endoproteinase glutamic acid (Glu)-C, PsaE was cleaved to 7.1- and 6.6-kD N-terminal fragments without significant cleavage of other subunits. Glu63 and Glu67, located near the C terminus of PsaE, were the most likely cleavage sites. At higher protease concentrations, the PsaE fragments were further cleaved and an N-terminal 9.8-kD PsaD fragment accumulated, demonstrating the accessibility of Glu residue(s) in the C-terminal domain of PsaD to the protease. Besides these major, primary cleavage products, several secondary cleavage sites on PsaD, PsaE, and PsaF were also identified. PsaF resisted proteolysis when PsaD and PsaE were intact. Glu88 and Glu124 of PsaF became susceptible to endoproteinase Glu-C upon extensive cleavage of PsaD and PsaE. Modification of PSI proteins with NHS-biotin and subsequent cleavage by endoproteinase Glu-C or thermolysin showed that the intact PsaE and PsaD, but not their major degradation products lacking C-terminal domains, were heavily biotinylated. Therefore, lysine-74 at the C terminus of PsaE was accessible for biotinylation. Similarly, lysine-107, or lysine-118, or both in PsaD could be modified by NHS-biotin.

Non-NASA Center↗

NASA NTRS1986

Generalized energetics for linear and nonlinear symmetric instabilities

The generalized energy conservation integral is applied to linear and nonlinear symmetric instabilities (SIs) and conditional symmetric instabilities (CSIs) in uniform and nonuniform basic states. The generalized energy conservation integral for linear SI in nonuniform basic state is refined to include initial thermal-inertial perturbations. A generalized conservation integral is derived for a nonlinear SI with uniform and nonuniform basic states. The integral reveals that: (1) the stability conditions of nonlinear and linear SI are not the same; (2) when the basic state is symmetrically stable the nonlinear evolution of a symmetric disturbance is energetically bounded by its initial energy; and (3) when the basic state is symmetrically unstable the growth of the symmetric perturbation is approximated by the linear SI theory. Precipitative heating is incorporated into the generalized energy integral for nonlinear CSI; the relation between nonlinear evolution of a CSI circulation and precipitative heating is examined.

Xu, Q.↗

NASA NTRS1986

Conditional symmetric instability and mesoscale rainbands

The linear theory of conditional symmetric instability (CSI) is re-examined in a rigorous framework. In comparison with symmetric instability a new feature of CSI is that the moist updraught tends to be narrow, as with conditional buoyancy instability (CBI). As the width of the moist updraught varies from its tolerance maximum to infinitesimal, the inviscid growth rate increases from zero to its maximum and the slope of the moist updraught increases from the absolute momentum surface to the moist most unstable surface. The fact that CSI circulations absorb energy from the basic shear and moist thermal field but lose energy to the dry basic thermal field is responsible for the narrow and slant feature of the moist updraught. When a bulk viscosity is accounted for, the most rapidly growing CSI modes bear a qualitative resemblance to some observed rainbands. The stability criterion of viscous CSI also shows a better comparison with observational data than inviscid CSI. The linear CSI theory here predicts that the isolated mode is preferred to other non-isolated (periodic or irregular spacing) modes. The preference of non-isolated modes is speculated to occur in the nonlinear stage.

Xu, Q.↗

NASA NTRS1985

The nature of symmetric instability and its similarity to convective and inertial instability

It is presently noted that, even for the case of fully nonlinear viscous motion, there exists a local similarity among symmetric, buoyancy or convective, and inertial instabilities. The most unstable slope angles for symmetric instability are analyzed through consideration of parcel energetics; the results obtained suggest that qualitatively conditional symmetric instability circulations will be slantwise, and will lie between the moist and most unstable and the dry and least stable slopes of the basic state.

Xu, Q.↗

NASA NTRS1985

The nature of symmetric instability and its similarity to convective and inertial instability

It is shown that there exists a local similarity among SI (Symmetric Instability), BI (Buoyancy or Convective Instability), and II (Inertial Instability) even for fully nonlinear viscous motion. The most unstable slope angles for SI and Moist SI motions are analyzed based on parcel energetics. These considerations also suggest qualitatively that CSI (Conditional SI) circulations will be slantwise and lie between the moist most unstable slope and dry least stable slope of the basic state.

Xu, Q.↗

NASA NTRS1984

Wave CISK and mesoscale convective systems

An examination is conducted of the basic assumptions of conventional wave-CISK theory, against observational data on mesoscale-organized convective systems. In response to the inadequacy of the quasi-equilibrium assumption, a nonequilibrium model of clouds actively responding to mesoscale low level mass fluxes in a preexisting and conditionally unstable atmosphere is formulated. The structures obtained for the propagating and growing waves are noted to be comparable with observations of midlatitude mesoscale convective systems. An examination is made of the energetics of this modified wave-CISK model, in order to deepen understanding of its instability and scale selection.

Xu, Q.↗