Biomimetic Frustrated Lewis Pair Catalysts for Hydrogenation of CO to Methanol at Low Temperatures
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1,1-addition↗
Engineering topics
Xie, Xiaofeng
Publications and source records attributed to Xie, Xiaofeng.
Sample Preparation Methods for Targeted Single-Cell Proteomics
We compared three cell isolation and two proteomic sample preparation methods for single-cell and near-single-cell analysis. Whole blood was used to quantify hemoglobin (Hb) and glycated-Hb (gly-Hb) in erythrocytes using targeted mass spectrometry and stable isotope-labeled standard peptides. Each method differed in cell isolation and sample preparation as follows: 1) FACS and automated preparation in one-pot for trace samples (autoPOTS); 2) limited dilution via microscopy and a novel rapid one-pot sample preparation method that circumvented the need for the solid-phase extraction, low-volume liquid handling instrumentation and humidified incubation chamber; and 3) CellenONE-based cell isolation and the same one-pot sample preparation method used for limited dilution. Only the CellenONE device routinely isolated single-cells from which Hb was measured to be 540–660 amol per red blood cell (RBC), which was comparable to the calculated SI reference range for mean corpuscular hemoglobin (390–540 amol/RBC). FACSAria sorter and limited dilution could routinely isolate single-digit cell numbers, to reliably quantify CMV-Hb heterogeneity. Finally, we observed that repeated measures, using 5–25 RBCs obtained from N = 10 blood donors, could be used as an alternative and more efficient strategy than single RBC analysis to measure protein heterogeneity, which revealed multimodal distribution, unique for each individual.
59 BASIC BIOLOGICAL SCIENCES↗
Is the polarization of the C$=$C bond imperative for bifunctional outer-sphere C$=$C hydrogenation?
Understanding controlling factors is important for the development of bifunctional outer-sphere C$=$C hydrogenations. In this study, a dominant view is that the polarization of C$=$C bonds is imperative for these reactions. However, the present comparative DFT study suggests that the polarization of C$=$C bonds is not the controlling factor. Instead, the “push–pull” type π-conjugative effect can decrease activation barriers and contribute to outer-sphere bifunctional C$=$C bond hydrogenations. What is more, this study shows the feasibility of the asymmetric bifunctional outer-sphere C$=$C hydrogenation.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗