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Taylor, G. R.

Publications and source records attributed to Taylor, G. R..

At least 37 records · Page 2
All sourcesNASA NTRSDOE OSTI
NASA NTRS1977

Cell biology experiments conducted in space

A review of cell biology experiments conducted during the first two decades of space flight is provided. References are tabulated for work done with six types of living test system: isolated viruses, bacteriophage-host, bacteria, yeasts and filamentous fungi, protozoans, and small groups of cells (such as hamster cell tissue and fertilized frog eggs). The general results of studies involving the survival of cells in space, the effect of space flight on growing cultures, the biological effects of multicharged high-energy particles, and the effects of space flight on the genetic apparatus of microorganisms are summarized. It is concluded that cell systems remain sufficiently stable during space flight to permit experimentation with models requiring a fixed cell line during the space shuttle era.

Taylor, G. R.↗
NASA NTRS1977

Survey of cell biology experiments in reduced gravity

The effects of spaceflight on terrestrial cell systems are discussed. With some important exceptions, static cell systems carried aboard U.S.A. and U.S.S.R. space flights have failed to reveal space related anomalies. Some sophisticated devices which were developed for viewing directly, or continuously recording, the growth of cells, tissue cultures and eggs in flight, are described and the results summarized. The unique presence of high energy, multicharged (HZE) particles and full-range ultraviolet irradiation in space prompted evaluation of the response of single cells to these factors. Summary results and general conclusions are presented. Potential areas of research in future space flights are identified.

Taylor, G. R.↗
NASA NTRS1977

Growth-rate periodicity of Streptomyces levoris during space flight

Streptomyces levoris provides a suitable biological test system to investigate the effects of space flight on the rhythms of vegetative and spore phase characteristics of both growth-rate periodicity and culture morphology during the pre-, in-, and post-flight periods of the Apollo-Soyuz Test Project. The objectives of the American participation were to study the effects of space flight on the biorhythms of Streptomyces levoris based on a comparison of the growth-rate periodicity of the vegetative and spore phase within each culture, to examine the possible alteration of spore morphology and development by SEM, and to compare the effects of a 12-hr phase shift on the periodic growth characteristics of this microorganism in cultures which were exchanged during the joint activities of the space flight. No uniform differences in the biorhythm of Streptomyces levoris during space flight were observed. It appears that the single most variable factor related to the experiment was the lack of temperature control for the space-flight specimens.

Rogers, T. D.↗
NASA NTRS1976

Joint inflight biomedical experiments performed during the ASTP spaceflight

Two joint inflight biomedical experiments were conducted during the unique Apollo-Soyuz Test Project (ASTP) spaceflight. One experiment evaluated rhythmicity of spore production of Streptomyces levoris. The other evaluated components of the infectious disease process by measuring alteration in: (1) the composition of the microbial population inhabiting USA and USSR crewmembers and spacecraft; (2) the ability of each crewmember's defense mechanism to resist infection; and (3) the ability of certain microorganisms to originate infections. These two experiments are described and the major results discussed.

Taylor, G. R.↗
NASA NTRS1976

The surface of Mars - The view from the Viking 1 lander

Imagery of the surface of Mars obtained by Viking 1 is analyzed. The lander is situated on the western slopes of the 5-km deep Chryse Planitia depression, about 2 km higher than the floor. The topography is gently rolling. Angular rocks and small sand dunes are visible. There are very few craters; initial evaluations indicate that crater area densities are several orders of magnitude below saturation for crater sizes less than about 50 m. The presence of scour marks and of fine-grained deposits in some boulders indicates that some aeolian activity has occurred. Almost all the sky brightness can be attributed to scattering by particles present in the atmosphere. No signs of movement have been detected, consistent with the low seasonal winds recorded by meteorological instruments.

Mutch, T. A.↗
NASA NTRS1976

Microbiology studies in the Space Shuttle

Past space microbiology studies have evaluated three general areas: microbe detection in extraterrestrial materials; monitoring of autoflora and medically important species on crewmembers, equipment, and cabin air; and in vitro evaluations of isolated terrestrial species carried on manned and unmanned spaceflights. These areas are briefly reviewed to establish a basis for presenting probable experiment subjects applicable to the Space Shuttle era. Most extraterrestrial life detection studies involve visitations to other heavenly bodies. Although this is not applicable to the first series of Shuttle flights, attempts to capture meteors and spores in space could be important. Human pathogen and autoflora monitoring will become more important with increased variety among crewmembers. Inclusion of contaminated animal and plant specimens in the space lab will necessitate inflight evaluation of cross-contamination and infection potentials. The majority of Shuttle microbiology studies will doubtless fall into the third study area. Presence of a space lab will permit a whole range of experimentation under conditions similar to these experienced in earth-based laboratories. The recommendations of various study groups are analyzed, and probable inflight microbiological experiment areas are identified for the Life Sciences Shuttle Laboratory.

Taylor, G. R.↗
NASA NTRS1976

Medical microbiological analysis of Apollo-Soyuz test project crewmembers

The procedures and results of the Microbial Exchange Experiment (AR-002) of the Apollo-Soyuz Test Project are described. Included in the discussion of procedural aspects are methods and materials, in-flight microbial specimen collection, and preliminary analysis of microbial specimens. Medically important microorganisms recovered from both Apollo and Soyuz crewmen are evaluated.

Taylor, G. R.↗
NASA NTRS1976

Zone-forming fungi experiment MA-147

Streptomyces levoris was used as an experimental microorganism during the Apollo Soyuz Test Project to study specific biological considerations that may be influenced by space flight factors. Preflight, inflight, and postflight growth rates of the cultures were compared by photographing the specimens at regular intervals. Preliminary results based on visual comparison of the photographic data indicate that an increased growth rate occurred during space flight in two of eight flight specimens. The increased growth rate continued in the two specimens during the postflight period until termination of the experiment. Radiation effects may be responsible for the absence of spores in two areas of the last spore ring that was formed during the inflight period in one of the flight cultures; however, the radiation studies related to this experiment have not been completed. Distinct morphological differences in spore rings were observed when postflight spore rings were compared with inflight spore rings. Factors that are related to space flight recovery and reentry into earth gravity may have effected these alterations.

Rogers, T. D.↗
NASA NTRS1976

Microbial exchange experiment AR-002

Operational aspects associated with the experiment and the activities of medically important microorganisms recovered from the Apollo crewmen are evaluated. A variety of potential pathogens was recovered from each of the prime and backup crew members before and after flight. However, no disease events were reported. Candida albicans and Staphylococcus aureus were shown to be transferred from one crewmember to another during the flight. No other medically significant changes in the microbial population were observed.

Taylor, G. R.↗
NASA NTRS1975

The Viking Mars lander camera

The 7.3 kg cameras for the 1976 Viking Mars expedition feature an array of 12 silicon photodiodes, including six spectral bands for color and near-infrared imaging with an angular resolution of 0.12 deg and four focus steps for broadband imaging, with an improved angular resolution of 0.04 deg. The field of view in elevation ranges from 40 deg above to 60 deg below the horizon, and in azimuth ranges to 342.5 deg. The cameras are mounted 0.8 m apart to provide a stereo view of the area accessible to a surface sampler for biological and chemical investigations. The scanning rates are synchronized to the lander data transmission rates of 16000 bits per sec to the Viking orbiters as relay stations and 250 bits per sec directly to earth. However, image data can also be stored on a lander tape recorder. About 10 million bits of image data will be transmitted during most days of the 60-day-long mission planned for each lander.

Huck, F. O.↗
NASA NTRS1975

Physical dosimetric evaluations in the Apollo 16 microbial response experiment

Texts of high-energy multicharged particle, ultraviolet light, and galactic irradiation fluxes on exposed biological specimens aboard Apollo 16 are described. Passive nuclear track detectors, passive dosimeters, and thermoluminescent dosimeters, respectively, were used for the measurements. Biological phenomena studied ranged from lipolytic alpha toxin production in Bacillus thuringiensis to animal tissue invasion (of human hair by Trichophyton terrestre). Fluxes observed were lower than those recorded by detectors located in the Biostack and in the passive personnel dosimeters worn by the astronauts, suggesting a greater average shielding in the latter two environments.

Taylor, G. R.↗
NASA NTRS1975

Effects of solar ultraviolet radiations on Bacillus subtilis spores and T-7 bacteriophage

Spores of Bacillus subtilis HA 101 and the DNA polymerase I-defective mutant HA 101 (59)F were exposed to selected wavelengths of solar ultraviolet light and space vacuum during the return of Apollo 16. In addition, coliphage T-7 suspensions were exposed to solar ultraviolet radiation as part of the Microbial Response to Space Environment Experiment. Optical filters were employed to provide different energy levels at wavelengths 254 nm and 280 nm. Dose-response curves for lethal and mutagenic effects were compared with ground-based data. A close parallel was observed between the results of solar radiation and ground tests with spores of the two strains. However, significantly greater inactivation of T-7 bacteriophage was observed after exposure to solar ultraviolet radiation.

Spizizen, J.↗
NASA NTRS1974

Recovery of medically important microorganisms from Apollo astronauts

Microbiological samples were obtained from the crewmembers of the Apollo 13, 14, 15, 16, and 17 spaceflights. These specimens were analyzed for the presence of medically important microorganisms with Staphylococcus aureus, Pseudomonas aeruginosa, Tricophyton mentagrophytes, Tricophyton rubrum, and Candida albicans being discussed in detail. Preflight isolation of crewmembers was found to coincide with a complete absence of inflight disease events and is recommended for future spaceflights. No autoinfection response (microbial shock) occurred after any of the reported spaceflights.

Taylor, G. R.↗
NASA NTRS1973

Crew microbiology (DTO 71-19)

States of microbial imbalance as a result of human altitude chamber confinement occurred, for the most part, only in those genera and species of bacteria, yeast, and fungi which are classified as transients and are not part of the true indigenous flora of the crewmembers. Inasmuch as no crew illness events occurred and only subtle changes in the indigenous flora were noted, it appears that confinement of 56-days in a Skylab simulated environment does not mediate toward shifts in bacterial populations which have obvious clinical significance.

Wooley, B. C.↗