A rapid Orthopoxvirus purification protocol suitable for high-containment laboratories
Not Available
Hughes, Laura↗
Engineering topics
Smith, Scott
Publications and source records attributed to Smith, Scott.
aoscaps3w (00)
The cavity attenuated phase shift extinction monitor is an optical extinction spectrometer that measures aerosols, broadband scatterers and absorbers of light that interact with solar radiation and influence the Earth’s radiation balance. The CAPS PMex uses a visible-light-emitting diode (LED), a sampling cell incorporating two high-reflectivity mirrors centered at the wavelength of the LED, and a vacuum photodiode detector.
54 ENVIRONMENTAL SCIENCES↗
Proteomic Assessment of Fluid Shifts and Association with Visual Impairment and Intracranial Pressure in Twin Astronauts
BACKGROUND: Astronauts participating in long duration space missions are at an increased risk of physiological disruptions. The development of visual impairment and intracranial pressure (VIIP) syndrome is one of the leading health concerns for crew members on long-duration space missions; microgravity-induced fluid shifts and chronic elevated cabin CO2 may be contributing factors. By studying physiological and molecular changes in one identical twin during his 1-year ISS mission and his ground-based co-twin, this work extends a current NASA-funded investigation to assess space flight induced "Fluid Shifts" in association with the development of VIIP. This twin study uniquely integrates physiological and -omic signatures to further our understanding of the molecular mechanisms underlying space flight-induced VIIP. We are: (i) conducting longitudinal proteomic assessments of plasma to identify fluid regulation-related molecular pathways altered by long-term space flight; and (ii) integrating physiological and proteomic data with genomic data to understand the genomic mechanism by which these proteomic signatures are regulated. PURPOSE: We are exploring proteomic signatures and genomic mechanisms underlying space flight-induced VIIP symptoms with the future goal of developing early biomarkers to detect and monitor the progression of VIIP. This study is first to employ a male monozygous twin pair to systematically determine the impact of fluid distribution in microgravity, integrating a comprehensive set of structural and functional measures with proteomic, metabolomic and genomic data. This project has a broader impact on Earth-based clinical areas, such as traumatic brain injury-induced elevations of intracranial pressure, hydrocephalus, and glaucoma. HYPOTHESIS: We predict that the space-flown twin will experience a space flight-induced alteration in proteins and peptides related to fluid balance, fluid control and brain injury as compared to his pre-flight protein/peptide signatures. Conversely, the trajectory of these protein signatures will remain relatively constant in his ground based co-twin. METHODS: We are using proteomic and standard immunoelectrophoresis techniques to delineate the change in protein signatures throughout the course of a long duration space flight in relation to the development of VIIP. We are also applying a novel cell-based metaboloic organ system assay ("Organs on a Plate") to address how these circulating biomarkers affect physiological processes at the cellular and organ level which could result in VIIP symptoms. These molecular data will be correlated with physiological measures (eg. extra and intracellular fluid volume, vascular filling/flow patterns, MRI, and Optic Coherence Tomography. DISCUSSION: Pre- and in-flight data collection is in progress for the space-flown twin, and similar data have been obtained from the ground-based twin. Biosamples will be batch processed when received from ISS after the conclusion of the 1-year mission. Omic and Physiological measures from the twin astronauts will be compared to similar data being collected on twin subjects who participated in simulated microgravity study. bed rest study.
Rana, Brinda K.↗
Effect of Pre-Strain on Impact Energy Dissipation in Silicone Foam Using Frequency-Based Kolsky Bar Analyses.
Abstract not provided.
Sanborn, Brett↗
Meteorological services annual data report for 2015
This document presents the meteorological data collected at Brookhaven National Laboratory (BNL) by Meteorological Services (Met Services) for the calendar year 2015. The purpose is to publicize the data sets available to emergency personnel, researchers and facility operations. Met services has been collecting data at BNL since 1949. Data from 1994 to the present is available in digital format. Data is presented in monthly plots of one-minute data. This allows the reader the ability to peruse the data for trends or anomalies that may be of interest to them. Full data sets are available to BNL personnel and to a limited degree outside researchers. The full data sets allow plotting the data on expanded time scales to obtain greater details (e.g., daily solar variability, inversions, etc.).
54 ENVIRONMENTAL SCIENCES↗
Pre-strain effect on frequency-based impact energy dissipation through a silicone foam pad for shock mitigation [Pre-strain effect on the frequency response of shock mitigation through a silicone foam pad]
Silicone foams have been used in a variety of applications from gaskets to cushioning pads over a wide range of environments. Particularly, silicone foams are used as a shock mitigation material for shock and vibration applications. Understanding the shock mitigation response, particularly in the frequency domain, is critical for optimal designs to protect internal devices and components more effectively and efficiently. The silicone foams may be subjected to pre-strains during the assembly process which may consequently influence the frequency response with respect to shock mitigation performance. A Kolsky compression bar was modified with pre-compression capabilities to characterize the shock mitigation response of silicone foam in the frequency domain to determine the effect of pre-strain. Lastly, a silicone sample was also intentionally subjected to repeated pre-strain and dynamic loadings to explore the effect of repeated loading on the frequency response of shock mitigation.
36 MATERIALS SCIENCE↗
The 2014 Sandia Nonlinear Mechanics and Dynamics Summer Research Institute
A collaborative research institute was organized and held at Sandia Albuquerque for a period of six weeks. This research institute brought together researchers from around the world to work collaboratively on a set of research projects. These research projects included: developing experimental guidelines for studying variability and repeatability of nonlinear structures; decoupling aleatoric and epistemic uncertainty in measurements to improve dynamic predictions; a numerical round robin to assess the performance of five different numerical codes for modeling systems with strong nonlinearities; and an assessment of experimentally derived and numerically derived reduced order models. In addition to the technical collaborations, the institute also included a series of seminars given by both Sandians and external experts, as well as a series of tours and field trips to local places of scientific and engineering importance. This report details both the technical research and the programmatic organization of the 2014 Sandia Nonlinear Mechanics and Dynamics Summer Research Institute.
36 MATERIALS SCIENCE↗
Meteorological Services Annual Data Report for 2014
This document presents the meteorological data collected at Brookhaven National Laboratory (BNL) by Meteorological Services (Met Services) for the calendar year 2014. The purpose is to publicize the data sets available to emergency personnel, researchers and facility operations. Met services has been collecting data at BNL since 1949. Data from 1994 to the present is available in digital format. Data is presented in monthly plots of one-minute data. This allows the reader the ability to peruse the data for trends or anomalies that may be of interest to them. Full data sets are available to BNL personnel and to a limited degree outside researchers. The full data sets allow plotting the data on expanded time scales to obtain greater details (e.g., daily solar variability, inversions, etc.).
54 ENVIRONMENTAL SCIENCES↗
The Effects of Boundary Conditions Measurement Techniques and Excitation Type on Measurements of the Properties of Mechanical Joints.
Abstract not provided.
Smith, Scott↗
aoscaps (01)
The cavity attenuated phase shift extinction monitor is an optical extinction spectrometer that measures aerosols, broadband scatterers and absorbers of light that interact with solar radiation and influence the Earth’s radiation balance. The CAPS PMex uses a visible-light-emitting diode (LED), a sampling cell incorporating two high-reflectivity mirrors centered at the wavelength of the LED, and a vacuum photodiode detector.
54 ENVIRONMENTAL SCIENCES↗
ARM: Aerosol Observing System (AOS): Air Flow Data 3
Aerosol Observing System (AOS): Air Flow Data 3
54 ENVIRONMENTAL SCIENCES↗
aosflow3 (a1)
The aerosol observing system (AOS) is the primary platform for in situ aerosol measurements at Earth’s surface. Each AOS has a different complement of instruments, which are selected to ensure the best measurements at each deployment site. The AOS measures aerosol optical properties to better understand how particles interact with solar radiation and influence the Earth's radiation balance. These measurements are useful for calculating parameters used in radiative forcing calculations, such as the aerosol single scattering albedo, asymmetry parameter, mass scattering efficiency, and hygroscopic growth. Measurements made with the AOS form a long-term record at the ARM fixed sites. Shorter measurement records are available from mobile deployments in a wide variety of geographical regions. These measurements are valuable for: identifying long-term changes in aerosol properties identifying atmospheric processes controlling aerosol life cycle and their influence on clouds providing experimental data for developing and testing atmospheric climate models.
54 ENVIRONMENTAL SCIENCES↗