Use of amino acid racemization to investigate the metabolic activity of dormant microorganisms in Siberian permafrost
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Engineering topics
Publications and source records attributed to McDonald, G..
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In the Antarctic Dry Valleys, cryptoendolithic microbial communities occur within porous sandstone rocks. Current understanding of the mechanisms of physiological adaptation of these communities to the harsh Antarctic environment is limited, because traditional methods of studying microbial physiology are very difficult to apply to organisms with extremely low levels of metabolic activity. In order to fully understand carbon and nitrogen cycling and nutrient uptake in cryptoendolithic communities, and the metabolic costs that the organisms incur in order to survive, it is necessary to employ molecular geochemical techniques such as amino acid analysis in addition to physiological methods.
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This article describes the development of hollow cathode ion lasers and their use in constructing an ultraviolet micro-Raman spectrograph with native fluorescence imaging capability.
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The amino acid distributions in the Murchison carbonaceous chondrite, Mars meteorite ALH84001, and ice from the Allan Hills region of Antarctica are shown, using a multivariate technique known as Principal Component Analysis (PCA), to be statistically distinct from the average amino acid compostion of 101 terrestrial protein superfamilies.
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Fe(VI) in the form of ferrate salts, with FeO(sub 4)(sup 2-) anion, was studied for its spectral and oxidative properties, with the question of whether it might be a suitable analog of the Mars soil oxidant, proposed as a result of the Viking missions of the early 1970s.
The Viking Gas Chromatograph Mass Spectrometer failed to detect organic matter on Mars, and both the Viking Labeled Release and Gas Exchange experiments indicated a highly oxidizing reactive soil on Mars surface.