Engineering Papers⌕ Search

Engineering topics

Martin, Matthew

Publications and source records attributed to Martin, Matthew.

6 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking

β-Coronaviruses such as SARS-CoV-2 hijack coatomer protein-I (COPI) for spike protein retrograde trafficking to the progeny assembly site in endoplasmic reticulum-Golgi intermediate compartment (ERGIC). However, limited residue-level details are available into how the spike interacts with COPI. Here we identify an extended COPI binding motif in the spike that encompasses the canonical K-x-H dibasic sequence. This motif demonstrates selectivity for αCOPI subunit. Guided by an in silico analysis of dibasic motifs in the human proteome, we employ mutagenesis and binding assays to show that the spike motif terminal residues are critical modulators of complex dissociation, which is essential for spike release in ERGIC. αCOPI residues critical for spike motif binding are elucidated by mutagenesis and crystallography and found to be conserved in the zoonotic reservoirs, bats, pangolins, camels, and in humans. Collectively, our investigation on the spike motif identifies key COPI binding determinants with implications for retrograde trafficking.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS2019

Satellite Salinity Observing System: Recent Discoveries and the Way Forward

Advances in L-band microwave satellite radiometry in the past decade, pioneered by ESA's SMOS and NASA's Aquarius and SMAP missions, have demonstrated an unprecedented capability to observe global sea surface salinity (SSS) from space. Measurements from these missions are the only means to probe the very-near surface salinity (top cm), providing a unique monitoring capability for the interfacial exchanges of water between the atmosphere and the upper-ocean, and delivering a wealth of information on various salinity processes in the ocean, linkages with the climate and water cycle, including land-sea connections, and providing constraints for ocean prediction models. The satellite SSS data are complimentary to the existing in situ systems such as Argo that provide accurate depiction of large-scale salinity variability in the open ocean but under-sample mesoscale variability, coastal oceans and marginal seas, and energetic regions such as boundary currents and fronts. In particular, salinity remote sensing has proven valuable to systematically monitor the open oceans as well as coastal regions up to approximately 40 km from the coasts. This is critical to addressing societally relevant topics, such as land-sea linkages, coastal-open ocean exchanges, research in the carbon cycle, near-surface mixing, and air-sea exchange of gas and mass. In this paper, we provide a community perspective on the major achievements of satellite SSS for the aforementioned topics, the unique capability of satellite salinity observing system and its complementarity with other platforms, uncertainty characteristics of satellite SSS, and measurement versus sampling errors in relation to in situ salinity measurements. We also discuss the need for technological innovations to improve the accuracy, resolution, and coverage of satellite SSS, and the way forward to both continue and enhance salinity remote sensing as part of the integrated Earth Observing System in order to address societal needs.

Vinogradova, Nadya↗