Engineering topics

Knipe, Jennifer M.

Publications and source records attributed to Knipe, Jennifer M..

4 records

DOE OSTI2022

Flow-through electrode capacitive deionization cell

Disclosed here is a capacitive deionization device for removing ions from a target solution. The capacitive deionization device includes a first porous electrode, a second porous electrode, a first header plate, a second header plate, and a sealant. The second porous electrode is disposed below and spaced from the first porous electrode. The first header plate is disposed on the first porous electrode. The first header plate defines an input flow channel that is in fluidic communication with the first porous electrode. The second header plate is disposed below the second porous electrode. The second header plate defines an output flow channel that is in fluidic communication with the second porous electrode. The sealant is disposed between the first header plate and the second header plate and surrounds the first porous electrode and the second porous electrode.

Campbell, Patrick G.↗

DOE OSTI2022

Multi-material modeling of sorption-desorption processes with experimental validation

Not Available

36 MATERIALS SCIENCE↗

DOE OSTI2022

Composite 3D-printed reactors for gas absorption, purification, and reaction

A composite material for gas capture including CO 2 capture and capture of other gases. The composite material includes solid or liquid reactive material, filler material, and a gas-permeable polymer coating such that the reactive material forms micron-scale domains in the filler material.

Nguyen, Du T.↗

DOE OSTI2021

Immobilization of transgenic plant cells towards bioprinting for production of a recombinant biodefense agent

Abstract Transgenic rice cells ( Oryza sativa ) producing recombinant butyrylcholinesterase (BChE) as a prophylactic/therapeutic against organophosphate nerve agent poisoning, cocaine toxicity, and neurodegenerative diseases like Alzheimer's were immobilized in a polyethylene glycol‐based hydrogel. The cells were sustained for 14 days in the semi‐solid matrix, undergoing a growth phase from days 0–6, a BChE production phase in sugar‐free medium from days 6–12, and a growth/recovery phase from days 12–14. Throughout this period, the cells maintained similar viability to those in suspension cultures and displayed analogous sugar consumption trends. The rice cells in the hydrogel also produced a significant amount of active BChE, comparable to the levels produced in liquid cultures. A considerable fraction of this BChE was secreted into the media, allowing for easier product separation. To the best of our knowledge, this proof‐of‐concept is the first report of immobilization of recombinant plant cells for continuous production of high‐value heterologous proteins. This work serves as a foundation for further investigation towards plant cell bioprinting and the development of a simple, efficient, robust, modular, and potentially field‐deployable bioreactor system for the manufacture of biologics.

Varma, Anika↗