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Kim, S. H.

Publications and source records attributed to Kim, S. H..

60 records · Page 4
All sourcesNASA NTRSDOE OSTI
NASA NTRS2001

Antisense expression of an Arabidopsis ran binding protein renders transgenic roots hypersensitive to auxin and alters auxin-induced root growth and development by arresting mitotic progress

We cloned a cDNA encoding an Arabidopsis Ran binding protein, AtRanBP1c, and generated transgenic Arabidopsis expressing the antisense strand of the AtRanBP1c gene to understand the in vivo functions of the Ran/RanBP signal pathway. The transgenic plants showed enhanced primary root growth but suppressed growth of lateral roots. Auxin significantly increased lateral root initiation and inhibited primary root growth in the transformants at 10 pM, several orders of magnitude lower than required to induce these responses in wild-type roots. This induction was followed by a blockage of mitosis in both newly emerged lateral roots and in the primary root, ultimately resulting in the selective death of cells in the tips of both lateral and primary roots. Given the established role of Ran binding proteins in the transport of proteins into the nucleus, these findings are consistent with a model in which AtRanBP1c plays a key role in the nuclear delivery of proteins that suppress auxin action and that regulate mitotic progress in root tips.

Non-NASA Center↗
NASA NTRS1996

The Interstellar Polarization Feature Associated with the 2175 Angstrom Extinction Bump

The most successful model at fitting the wavelength dependence of interstellar extinction consists of two populations of bare silicate and graphite grains (Mathis, Rumpl & Nordsieck, (MRN)). The graphite grains are needed to fit the strong 2175 A extinction bump, with the silicates providing most of the smooth extinction seen at other wavelengths. From observations of the IR silicate absorption features, it was inferred that the silicate grains, non-spherical and aligned to the Galactic magnetic field, were responsible for the interstellar polarization. Aligned silicate grains make a very good fit to the smooth featureless UV polarization curves that comprise most of the observed sample. The lines of sight showing a UV polarization feature require something different, perhaps a second population of grains as do the fits to the extinction curve. The polarization wavelength dependence of HD 197770 can be fit by a MRN-like mixture of aligned bare silicate and graphite grains. However, none of the popular grain models including MRN envisaged an aligned bump grain population. It has been suggested that the extinction bump is not due to graphite but rather to a population of polycyclic aromatic hydrocarbons (PAHs). If the observed UV polarization features could be associated with the 2175 A bump then it would strengthen the argument that the bump must be due to grains rather than PAHs which are not likely to be aligned to the Galactic Magnetic field. The reality of ultraviolet polarization features has been confirmed by observations with ASTRO-2. The original detection toward HD 197770 has been confirmed and a new feature has been detected toward HD 147933-4. Both features have centroids lying close to 2175 A the location of the UV extinction bump. Two possibilities are considered for the source of the polarization bump, alignment of graphite grains responsible for the extinction bump or changes in the size distribution of the aligned silicate grains responsible for the continuum polarization. It seems the graphite grains are a more likely source of the polarization.

Clayton, Geoffrey C.↗
NASA NTRS1989

Phytochrome induces changes in the immunodetectable level of a wall peroxidase that precede growth changes in maize seedlings

The regulatory pigment phytochrome induces rapid and opposite growth changes in different regions of etiolated maize seedlings: it stimulates the elongation rate of coleoptiles and inhibits that of mesocotyls. As measured by a quantitative immunoassay, phytochrome also promotes rapid and opposite changes in the extractable content of a Mr 98,000 anionic isoperoxidase in the cell walls of these same organs: it induces a decrease of this peroxidase in coleoptiles and an increase in mesocotyls. The peroxidase changes precede the growth changes. As measured by video stereomicroscopy or a position transducer, red light (R), which photoactivates phytochrome, stimulates coleoptile elongation with a lag of about 15-20 min and suppresses mesocotyl growth with a lag of 45-50 min. R also induces a 50% reduction in the extractable level of the anionic peroxidase in coleoptile walls in less than 10 min and a 40% increase in the level of this peroxidase in mesocotyl walls within 30 min. Ascorbic acid, an inhibitor of peroxidase activity, blocks the effects of R on mesocotyl section growth. These results are relevant to hypotheses that postulate that certain wall peroxidases can participate in light-induced changes in growth rate by their effects on wall extensibility.

Non-NASA Center↗
NASA NTRS1988

Production and characterization of monoclonal antibodies to wall-localized peroxidases from corn seedlings

A library of 22 hybridomas, which make antibodies to soluble wall antigens from the coleoptiles and primary leaves of etiolated corn (Zea mays L.) seedlings, was raised and cloned three times by limit dilution to assure monoclonal growth and stability. Two of these hybridomas made immunoglobulin G antibodies, designated mWP3 and mWP19, which both effectively immunoprecipitated peroxidase activity from crude and partially purified preparations of wall peroxidases. Direct peroxidase-binding assays revealed that both antibodies bound enzymes with peroxidase activity. As judged by immunoblot analyses, mWP3 recognized a Mr 98,000 wall peroxidase with an isoelectric point near 4.2, and mWP19 recognized a Mr 58,000 wall peroxidase. Immunogold localization studies showed both peroxidases are predominately in cell walls.

NASA Discipline Number 40-10↗
NASA NTRS1974

Three-dimensional tertiary structure of yeast phenylalanine transfer RNA

Results of an analysis and interpretation of a 3-A electron density map of yeast phenylalanine transfer RNA. Some earlier detailed assignments of nucleotide residues to electron density peaks are found to be in error, even though the overall tracing of the backbone conformation of yeast phenylalanine transfer RNA was generally correct. A new, more comprehensive interpretation is made which makes it possible to define the tertiary interactions in the molecule. The new interpretation makes it possible to visualize a number of tertiary interactions which not only explain the structural role of most of the bases which are constant in transfer RNAs, but also makes it possible to understand in a direct and simple fashion the chemical modification data on transfer RNA. In addition, this pattern of tertiary interactions provides a basis for understanding the general three-dimensional folding of all transfer RNA molecules.

Kim, S. H.↗