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Kim, Han

Publications and source records attributed to Kim, Han.

15 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Virus‐Based Pyroelectricity

Abstract The first observation of heat‐induced electrical potential generation on a virus and its detection through pyroelectricity are presented. Specifically, the authors investigate the pyroelectric properties of the M13 phage, which possesses inherent dipole structures derived from the noncentrosymmetric arrangement of the major coat protein (pVIII) with an α‐helical conformation. Unidirectional polarization of the phage is achieved through genetic engineering of the tail protein (pIII) and template‐assisted self‐assembly techniques. By modifying the pVIII proteins with varying numbers of glutamate residues, the structure‐dependent tunable pyroelectric properties of the phage are explored. The most polarized phage exhibits a pyroelectric coefficient of 0.13 µC m −2 °C −1 . Computational modeling and circular dichroism (CD) spectroscopy analysis confirm that the unfolding of α‐helices within the pVIII proteins leads to changes in phage polarization upon heating. Moreover, the phage is genetically modified to enable its pyroelectric function in diverse chemical environments. This phage‐based approach not only provides valuable insights into bio‐pyroelectricity but also opens up new opportunities for the detection of various viral particles. Furthermore, it holds great potential for the development of novel biomaterials for future applications in biosensors and bioelectric materials.

Kim, Han↗
DOE OSTI2022

An anti-PD-1–GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy

Costimulatory receptors such as glucocorticoid-induced tumor necrosis factor receptor–related protein (GITR) play key roles in regulating the effector functions of T cells. In human clinical trials, however, GITR agonist antibodies have shown limited therapeutic effect, which may be due to suboptimal receptor clustering-mediated signaling. To overcome this potential limitation, a rational protein engineering approach is needed to optimize GITR agonist-based immunotherapies. Here we show a bispecific molecule consisting of an anti-PD-1 antibody fused with a multimeric GITR ligand (GITR-L) that induces PD-1-dependent and FcγR-independent GITR clustering, resulting in enhanced activation, proliferation and memory differentiation of primed antigen-specific GITR + PD-1 + T cells. The anti-PD-1–GITR-L bispecific is a PD-1-directed GITR-L construct that demonstrated dose-dependent, immunologically driven tumor growth inhibition in syngeneic, genetically engineered and xenograft humanized mouse tumor models, with a dose-dependent correlation between target saturation and Ki67 and TIGIT upregulation on memory T cells. Anti-PD-1–GITR-L thus represents a bispecific approach to directing GITR agonism for cancer immunotherapy.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2021

Moisture-induced autonomous surface potential oscillations for energy harvesting

A variety of autonomous oscillations in nature such as heartbeats and some biochemical reactions have been widely studied and utilized for applications in the fields of bioscience and engineering. Here, we report a unique phenomenon of moisture-induced electrical potential oscillations on polymers, poly([2-(methacryloyloxy)ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide-co-acrylic acid), during the diffusion of water molecules. Chemical reactions are modeled by kinetic simulations while system dynamic equations and the stability matrix are analyzed to show the chaotic nature of the system which oscillates with hidden attractors to induce the autonomous surface potential oscillation. Using moisture in the ambient environment as the activation source, this self-excited chemoelectrical reaction could have broad influences and usages in surface-reaction based devices and systems. As a proof-of-concept demonstration, an energy harvester is constructed and achieved the continuous energy production for more than 15,000 seconds with an energy density of 16.8 mJ/cm 2 . A 2-Volts output voltage has been produced to power a liquid crystal display toward practical applications with five energy harvesters connected in series.

42 ENGINEERING↗
NASA NTRS2019

Estimation of Preferred Work Envelope from Simulated Functional Tasks in a Spacesuit

Spacesuits allow humans to function in an incredibly harsh environment. However, they introduce some restrictions to human capabilities. In general, crewmembers in a spacesuit have a restricted maximal reach envelope, reduced field of view, and reduced tactility. When tasks and interfaces are being designed, they need to take into account the restrictions associated with working in an Extravehicular Activity (EVA) suit.

Benson, Elizabeth↗
NASA NTRS2019

Assessment of Upper Body Posture and Shape with Garment-Embedded Flexible Strain Sensors

Spacesuits are critical to performing spacewalks or EVA, however can increase injury risks due to - Spacesuit Fit Concerns: Astronauts come from a diverse population; Improper suit fit will result in persistent contact and mechanical pressure on the body. - Altered Biomechanics: Mechanical constraints and pressurization can make it difficult due to - Reduced range of motion, Reduced strength capability. To improve suit design and mitigate injuries, kinematic and geometric measurements are required during EVA evaluations - Human motions inside a spacesuit may not coincide with the suit motions: Spacesuit configurations do not exactly match with human anatomical joints; Gaps/paddings inside the suit induce lags between human motion and suit motion - Conventional motion capture or 3-D scanning techniques are difficult to use to measure internal motion: Optical occlusions; Volume restrictions; Ferrous magnetic interference.

Vu, Linh↗
NASA NTRS2017

Assessment of Suited Reach Envelope in an Underwater Environment

Predicting the performance of a crewmember in an extravehicular activity (EVA) space suit presents unique challenges. The kinematic patterns of suited motions are difficult to reproduce in gravity. Additionally, 3-D suited kinematics have been practically and technically difficult to quantify in an underwater environment, in which crewmembers are commonly trained and assessed for performance. The goal of this study is to develop a hardware and software system to predictively evaluate the kinematic mobility of suited crewmembers, by measuring the 3-D reach envelope of the suit in an underwater environment. This work is ultimately aimed at developing quantitative metrics to compare the mobility of the existing Extravehicular Mobility Unit (EMU) to newly developed space suit, such as the Z-2. The EMU has been extensively used at NASA since 1981 for EVA outside the Space Shuttle and International Space Station. The Z-2 suit is NASA's newest prototype space suit. The suit is comprised of new upper torso and lower torso architectures, which were designed to improve test subject mobility.

Kim, Han↗
NASA NTRS2016

A Parametric Model of Shoulder Articulation for Virtual Assessment of Space Suit Fit

Goal of space human factors analyses: Place the highly variable human body within these restrictive physical environments to ensure that the entire anticipated population can live, work, and interact. Space suits are a very restrictive space and if not properly sized can result in pain or injury. The highly dynamic motions performed while wearing a space suit often make it difficult to model. Limited human body models do not have much allowance for customization of anthropometry and representation of the population that may wear a space suit.

Young, Karen↗