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Jared Shimada

Publications and source records attributed to Jared Shimada.

5 records
All sourcesNASA NTRSDOE OSTI
NASA NTRSDate not supplied

Europa Luminescence Microscope

The Europa Luminescence Microscope (ELM) is an automated fluorescence and dark-field mi-croscope designed to meet key objectives defined in the NASA Europa Lander Study Report, includ-ing the identification and characterization of morphological biosignatures. ELM’s heritage stems from a 2U cubesat fluorescence microscope, the Fluorescence Analysis for In-situ Research (FLAIR) im-ager, designed and built at NASA Ames Research Center, for the autonomous study of microbial bi-ology in low Earth orbit. For the ELM implementation, a sample is autonomously manipulated with a microfluidic system using in-line filter sets to capture successively smaller particles on 10, 1.0, and 0.1 µm pore-size filters for imaging. For darkfield imaging, ELM uses ultraviolet and visible light to image organic and inorganic structures with submicron resolution. The ability to detect structural and chemical biosignatures as small as 0.2 µm in size is achieved by imaging native fluorescence and us-ing fluorescence microscopy stains to identify key molecular and structural indicators of microbial life (proteins, lipids, nucleic acids). To excite fluorescence, ELM uses LEDs with wavelengths centered near 265, 370, 470, and 530 nm and five emission bands. The use of multiple excitation and emission wavelengths for native fluorescence imaging not only enables the detection of different molecular species, but also their rough classification. Excitation at 265 nm allows for the detection of smaller polyaromatic hydrocarbons (PAHs; 1-5 rings), aromatic amino acids, and proteins with little to no interference from mineral fluorescence, given proper emission band selection. 370 and 470 nm light excites increasingly larger PAH structures (e.g., coronene) and larger aromatic biomolecules that may be present (e.g., protective pigments). Similarly, inorganic fluorescence can be characterized and sep-arated from organic fluorescence, allowing the recognition and in some cases classification, of miner-als and other abiotic particles. ELM is based upon work supported by the NASA COLDTech and ICEE-2 programs.

Microscope↗
NASA NTRSDate not supplied

Sample Processing Instrumentation to Enable Lab-like Analysis of Lipids, Hydrocarbons, and Kerogen in situ

Introduction: Over the last decade, our team based out of NASA Ames Research Center has made advances in fluidic technologies to enable laboratory-grade processes in space environments. More recently, we have designed and developed two instruments that utilize sample processing steps to extract and purify organic molecules that are key targets in the search for life. The first instrument targets soluble lipids and hydrocarbons that have been preserved in soil or rock material for millions to billions of years called ExCALiBR (Extractor for Chemical Analysis of Lipid Biomarkers in Regolith). The second instrument called KAMELOT (Kerogen and Macromolecule Extractor Liberating Organics Thermolytically) targets kerogen or insoluble macromolecular material that reside in the residue left behind after solvent extraction. Together, these instruments can be coupled to existing flight sample acquisition and analytical instrumentation (e.g., mass spectrometers) to achieve our overall science goal of identifying molecular patterns and features that are diagnostic of how the organic matter was synthesized, either through biological or non-biological processes.

Mary Beth Wilhelm↗
NASA NTRSDate not supplied

NASA Ames Flight Instrument Group - MARS Exploration Efforts Overview

The NASA Ames Flight Instrument Group (FIG) MARS development activities include Aeolus, an integrated multi-probe mission to observe surface and atmospheric forcing and general circulation of Mars; the Mars Sonic Anemometer (MSA), a sonic-based, in-situ wind measuring instrument and the Saltation Sensor to detect the critical wind stress threshold for sand motion, which is believed to be a key aspect of dust lifting at Mars. These efforts are readying the instruments to fly, and their measurements are regarded as data of high scientific priority for future flight missions. Each of these efforts focuses on addressing a measurement or capability considered critical within the Agency and traceable to the decadal survey and MEPAG Goals. As we elevate the Technology Readiness Level (TRL) of these instruments to 6 through rigorous environmental testing, and participate in forming mission concepts, we make them viable for inclusion in flight mission proposals. Below, we outline FIG's highest priority advancements for Mars exploration.

Mars↗
NASA NTRSDate not supplied

NASA Ames Flight Instrument Group - MARS Exploration Efforts Overview

The NASA Ames Flight Instrument Group (FIG) MARS development activities include Aeolus, an integrated multi-probe mission to observe surface and atmospheric forcing and general circulation of Mars; the Mars Sonic Anemometer (MSA), a sonic-based, in-situ wind measuring instrument and the Saltation Sensor to detect the critical wind stress threshold for sand motion, which is believed to be a key aspect of dust lifting at Mars. These efforts are readying the instruments to fly, and their measurements are regarded as data of high scientific priority for future flight missions. Each of these efforts focuses on addressing a measurement or capability considered critical within the Agency and traceable to the decadal survey and MEPAG Goals. As we elevate the Technology Readiness Level (TRL) of these instruments to 6 through rigorous environmental testing, and participate in forming mission concepts, we make them viable for inclusion in flight mission proposals. Below, we outline FIG's highest priority advancements for Mars exploration.

Mars↗