Engineering topics

Jackson, Joseph

Publications and source records attributed to Jackson, Joseph.

4 records

DOE OSTI2025

405 nm light microbicidal efficacy on Treponema pallidum spiked in ex vivo human platelets

Abstract Pathogen reduction technologies using chemicals and or UV light have been demonstrated to improve the safety of ex vivo platelets from infectious diseases. However, UV light exposure also may affect the treated products, depending on wavelength and exposure. Alternatively, visible spectra 405 nm violet-blue light has broad-spectrum microbicidal activity. Here we tested the effect of 405 nm light onTreponema pallidum, the bacterium that causes syphilis. We contaminated platelets stored in plasma with two treponemal concentrations (low and high titers) and treated an aliquot with 270 J/cm 2 dose (irradiance = 15 mW/cm 2 ) of 405 nm light while another aliquot remained untreated. Next, we inoculated intradermally an aliquot of both samples into rabbits. Rabbits inoculated with untreated samples developed syphilis while animals inoculated with light-treated samples did not. Thus, inactivation was demonstrated to the limit of detection of the bioassay. We estimated > 2 log 10 and > 4 log 10 reduction in the low and high dose studies, respectively. These results provide proof-of-concept that 405 nm light is effective in reducing syphilis risk in ex vivo platelets.

Science & Technology - Other Topics↗

DOE OSTI2024

405 nm violet-blue light inactivates hepatitis C cell culture virus (HCVcc) in ex vivo human platelet concentrates and plasma

Abstract Added safety measures coupled with the development and use of pathogen reduction technologies (PRT) significantly reduces the risk of transfusion-transmitted infections (TTIs) from blood products. Current approved PRTs utilize chemical and/or UV-light based inactivation methods. While the effectiveness of these PRTs in reducing pathogens are well documented, these can cause tolerable yet unintended consequences on the quality and efficacy of the transfusion products. As an alternative to UV-based approaches, we have previously demonstrated that 405 nm violet-blue light exposure successfully inactivates a variety of pathogens, including bacteria, parasites, and viruses, in both platelet concentrates (PCs) and plasma. Herein, we show that 405 nm light treatment effectively inactivates hepatitis C cell culture virus (HCVcc) by up to ~ 3.8 log10 in small volumes of a variety of matrices, such as cell culture media, PBS, plasma, and PCs with 27 J/cm 2 of light exposure, and total inactivation of HCVcc after 162 J/cm 2 light exposure. Furthermore, we demonstrate that carry-over of media supplemented with fetal bovine serum enhances the production of reactive oxygen species (ROS), providing mechanistic insights to 405 nm light-mediated viral inactivation. Overall, 405 nm light successfully inactivates HCVcc, further strengthening this method as a novel PRT for platelets and plasma.

Science & Technology - Other Topics↗

NASA NTRS2020

Progress on Gust Load Alleviation Wind Tunnel Experiment and Aeroservoelastic Model Validation for a Flexible Wing with Variable Camber Continuous Trailing Edge Flap System

This paper discusses a wind tunnel experiment of active gust load alleviation of a flexible wing which took place at University of Washington (UW) in 2019. The experiment performed under a NASA SBIR contract with Scientific Systems Company, Inc (SSCI). The objective of the experiment is to demonstrate active controls of the Variable Camber Continuous Trailing Edge Flap (VCCTEF) system for gust load alleviation and real-time drag optimization. The wind tunnel model is a 8.2% sub-scale Common Research Model (CRM) wing. The wing structure is designed to provide a substantial degree of flexibility to represent that of a modern high-aspect ratio wing. Eight active control surfaces are employed in the VCCTEF. A new gust generator system was designed and installed by UW under a sub-contract with SSCI. The first test entry started in July 2019 and ended in September 2019. During this test entry, many significant issues were found with the hardware and software. The significant issues with the servos prevented the test objective from being completed. A follow-up second test entry in 2020 is being planned. The wing system is being repaired by SSCI. This paper reports on the progress of this experimental effort and the aeroservoelastic (ASE) model validation which was conducted during the test entry.

Nguyen, Nhan↗

NASA NTRS1999

Current and Future Developments in Air Traffic Control

Current and future energy demands, end uses, and cost used to characterize typical applications services in the industrial sector of the United States are examined. A review and evaluation of existing industrial energy data bases was undertaken to assess their potential for supporting SERI research market suitability analysis; (2) market development; (3) end use matching; (4) industrial application studies; and (5) identification of cost and performance goals for solar systems and typical information requirements for industrial energy end use. The focus was on fuels and electric energy used for heat and power purchased by the manufacturing subsector and listed by 2, 3, and 4 digit SIC, primary fuel. The effects of federal and state industrial energy conservation programs on future industrial sector demands were assessed.

Jackson, Joseph↗