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Daughtry, Craig S. T.

Publications and source records attributed to Daughtry, Craig S. T..

Diurnal and Seasonal Variations in Chlorophyll Fluorescence Associated with Photosynthesis at Leaf and Canopy Scales

There is a critical need for sensitive remote sensing approaches to monitor the parameters governing photosynthesis, at the temporal scales relevant to their natural dynamics. The photochemical reflectance index (PRI) and chlorophyll fluorescence (F) offer a strong potential for monitoring photosynthesis at local, regional, and global scales, however the relationships between photosynthesis and solar induced F (SIF) on diurnal and seasonal scales are not fully understood. This study examines how the fine spatial and temporal scale SIF observations relate to leaf level chlorophyll fluorescence metrics (i.e., PSII yield, YII and electron transport rate, ETR), canopy gross primary productivity (GPP), and PRI. The results contribute to enhancing the understanding of how SIF can be used to monitor canopy photosynthesis. This effort captured the seasonal and diurnal variation in GPP, reflectance, F, and SIF in the O2A (SIFA) and O2B (SIFB) atmospheric bands for corn (Zea mays L.) at a study site in Greenbelt, MD. Positive linear relationships of SIF to canopy GPP and to leaf ETR were documented, corroborating published reports. Our findings demonstrate that canopy SIF metrics are able to capture the dynamics in photosynthesis at both leaf and canopy levels, and show that the relationship between GPP and SIF metrics differs depending on the light conditions (i.e., above or below saturation level for photosynthesis). The sum of SIFA and SIFB (SIFA+B), as well as the SIFA+B yield, captured the dynamics in GPP and light use efficiency, suggesting the importance of including SIFB in monitoring photosynthetic function. Further efforts are required to determine if these findings will scale successfully to airborne and satellite levels, and to document the effects of data uncertainties on the scaling.

Campbell, Petya K.

Coming of Age: Polarization as a Probe of Plant Canopy Water Status

We tested the hypothesis that the relative water content (RWC) of the sunlit leaves in a plant canopy may be estimated from polarized canopy imagery. Recently (IGARSS, July 27-31, 2015, Milan, Italy), we reported the results of laboratory polarization measurements of single detached leaves during dry down. We found that RWC was linearly related to the ratio of the reflectance of the interior of the leaf and the leaf transmittance. Here we report application of the laboratory results to estimate RWC for sunlit leaves in a plant canopy. Using a commercial-off-the-shelf (COTS) Nikon 810 camera with Nikkor 300 mm lens and Polaroid type HN-22 linear polarizer, we photographed in the principle plane a plant canopy displaying a gradient of water stress and collected, at each of multiple points along the gradient, two images, one with the polarization filter oriented for maximum scene response and a second with the filter oriented for minimum scene response. We converted the digital values in the two images to reflectance factor with reference to images of a white, flat, horizontal Spectralon surface. We classified the polarization imagery, identifying reflecting leaves, transmitting leaves, other sunlit vegetation and shadows. For each image pair we normalized the leaf internal reflectance by dividing by the cosine of the angle of incidence of the sunlight on the leaf, selected the leaf maximum transmittance in the scene and divided to obtain the ratio reflectance/transmittance, which we compared with leaf RWC. We determined the leaf relative water content by harvesting a section of leaf and immediately placing it in a sealed container in an ice chest. Later in the laboratory the leaf sample was weighed, rehydrated, weighed, dried and again weighed. RWC was determined using the standard formula. Our experimental results support our hypothesis, suggesting that the RWC of sunlit leaves in a plant canopy may be estimated from analysis of polarization imagery collected by a COTS camera system. Unlike remotely sensed estimates of canopy equivalent water thickness, our estimates of the RWC of sunlit canopy leaves provide leaf physiological information. We propose RWC estimates based upon sunlit leaves are more relevant to assessing the water status of a plant canopy than would be RWC estimates based upon large FOV canopy measurements.

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Spectral Indices to Monitor Nitrogen-Driven Carbon Uptake in Field Corn

Climate change is heavily impacted by changing vegetation cover and productivity with large scale monitoring of vegetation only possible with remote sensing techniques. The goal of this effort was to evaluate existing reflectance (R) spectroscopic methods for determining vegetation parameters related to photosynthetic function and carbon (C) dynamics in plants. Since nitrogen (N) is a key constituent of photosynthetic pigments and C fixing enzymes, biological C sequestration is regulated in part by N availability. Spectral R information was obtained from field corn grown at four N application rates (0, 70, 140, 280 kg N/ha). A hierarchy of spectral observations were obtained: leaf and canopy with a spectral radiometer; aircraft with the AISA sensor; and satellite with EO-1 Hyperion. A number of spectral R indices were calculated from these hyperspectral observations and compared to geo-located biophysical measures of plant growth and physiological condition. Top performing indices included the R derivative index D730/D705 and the normalized difference of R750 vs. R705 (ND705), both of which differentiated three of the four N fertilization rates at multiple observation levels and yielded high correlations to these carbon parameters: light use efficiency (LUE); C:N ratio; and crop grain yield. These results advocate the use of hyperspectral sensors for remotely monitoring carbon cycle dynamics in managed terrestrial ecosystems.

Corp, Lawrence A.

Method for determining surface coverage by materials exhibiting different fluorescent properties

An improved method for detecting, measuring, and distinguishing crop residue, live vegetation, and mineral soil is presented. By measuring fluorescence in multiple bands, live and dead vegetation are distinguished. The surface of the ground is illuminated with ultraviolet radiation, inducing fluorescence in certain molecules. The emitted fluorescent emission induced by the ultraviolet radiation is measured by means of a fluorescence detector, consisting of a photodetector or video camera and filters. The spectral content of the emitted fluorescent emission is characterized at each point sampled, and the proportion of the sampled area covered by residue or vegetation is calculated.

Chappelle, Emmett W.

Scene shadow effects on multispectral response

Spectral responses measured from nonuniform balsam fir canopies with varying amounts and locations of shadows are examined in order to study the effects of scene shadows on spectral response. The behavior of canopy reflectance and spectral band transformations, normalized difference (ND), and greenness for a variety of solar zenith angles and background types are analyzed. The use of vegetation indices for monitoring vegetation is studied in terms of shadowing. It is observed that the red and near-IR reflectance factors decrease as the amount of scene shadows increase; the ND increases with increasing scene shadows; changing shadow amounts have little effect on greenness; and the ND from 'pure' pixels increases as the shadows cast by adjacent vegetation canopies increases.

Ranson, K. Jon