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Daniella Saetta

Publications and source records attributed to Daniella Saetta.

The membrane bioreactor (MBR): A hybrid technology for bioregenerative wastewater treatment and resource recovery in space

Extraplanetary surface habitat life support systems (LSS) on the Moon and Mars, as well as long-duration space travel, will require novel capabilities to withstand anticipated unique, harsh conditions. In order to provide safe, habitable environments for the crew, water purification and waste processing systems will be required to treat all sources of water (condensate, Sabatier, urine, hygiene, fecal, food waste) in order to achieve the necessary levels of recovery needed to sustain life over the long-duration missions. The ability to recycle organic wastes creates an opportunity to recover critical elements (e.g., C, H, O, N, P) for subsequent food production, water purification, and atmospheric regeneration. Bioregenerative systems mimic functions of nature in engineered systems, or bioreactors, utilizing combination of prokaryotes, eukaryotes and archaea. While these systems are commonly used on Earth for wastewater treatment, bioreactors for space travel face additional challenges. Terrestrial bioreactors often rely on gravitational settling of dense flocs and granules for cell retention. For micro- or partial-gravity environments, density differential alone will not be adequate for cell retention; a gravity-independent means for cell retention is crucial. The membrane bioreactor represents the state of the art in wastewater treatment. This hybrid system combines biological processes with membrane filtration to achieve performance beyond what each can accomplish individually. The complete cell retention in an MBR allows for the decoupling of hydraulic retention time (HRT) and solids retention time (SRT), which result in a high-thruput, compact, treatment system. The Bioregenerative Water Technology Team at NASA Kennedy Space Center and the University of South Florida has developed a bioregenerative platform based on the hybrid MBR technology. The overall architecture is compact, modular, flexible, and adaptable to mission evolutions. The main subsystems of the bioregenerative architecture include: 1) Anaerobic membrane bioreactor (AnMBR): Also termed the Organic processor assembly (OPA), the function of the AnMBR is to treat organic wastes such as fecal and food wastes. These wastes are characterized by a concentration of suspended solids comprised of carbohydrates, proteins and lipids. The assigned function of the AnMBR is to break down and covert suspended solids to biogas (methane, hydrogen and carbon dioxide), reduce effluent chemical oxygen demand (COD), liberate organically-bound nutrients, and remove pathogenic organisms. 2) Phototrophic membrane bioreactor (PMBR): The PMBR is comprised of a co-culture of microalgae and bacteria. The assigned function of the PMBR is to polish the permeate of the AnMBR to further remove dissolved organic carbon, manage nutrients (nitrogen transformation, load dampening), and perform air revitalization. 3) Food processor assembly (FPA): The FPA is a food production platform (prokaryotic or eukaryotic), fueled by outputs from the AnMBR, or PMBR. For the presentation, we will describe each step of the bioregenerative architecture, and present performance data from extended trials treating analog and real metabolic wastes.

Bioreactor↗

Evaluation of Long Term Microbial Regrowth in Slosh Water Tanks From the International Space Station

The NASA Launch Services Program (LSP) maintained the SPHERES-Slosh experiment aboard the International Space Station (ISS) between 2013 and 2019. The purpose of the Slosh experiment was to examine how liquids move inside fuel tanks in a microgravity environment. These tanks were similar to water storage tanks planned for use aboard future space systems, where large dormant periods between crew-use will provide similar conditions for biological growth or chemical leaching. The water within the SLOSH tanks remained undisturbed for over five years after testing concluded, providing a unique sample for stored water under microgravity conditions without prior protocols for microbial control such as sterilization or addition of biocides. The Slosh storage tanks were returned to Kennedy Space Center (KSC) aboard SpaceX CRS-18 mission in November 2019. Upon return of the tanks, the water within each tank was analyzed to determine how the water chemistry and biology changed during its tenure in microgravity. The data obtained and described within this publication provided a basis and reasoning for planning water storage and purification treatment methods aboard ISS, Gateway, and future space habitats. Results demonstrated that low microbial concentrations were present within the water, as expected since no biocide treatment was employed, yet no extensive biofilm formation was observed after 5 years even in the presence of microbial food sources such as the polycarbonate structure and food color additives. This experimentation demonstrates that future biofilm studies should be performed on this type of experimental setup with proper controls aboard ISS to examine microbial regrowth to improve microbial control within space water systems.

Luke B. Roberson↗

Alternative Treatment of Crew Wastewater Using a Hybrid Membrane Technology

Environmental Control and Life Support Systems (ECLSS) of future long-duration, deep-space human exploration missions should treat all “waste” streams for recovery and ideally by near closed-loop. Onboard the International Space Station (ISS), the Water Recovery System (WRS) does not treat urine and gray water to recover elements beyond water and utilizes physical-chemical technologies that operate with single-use and hazardous consumables. Urine offers a renewable source of nitrogen and other trace elements that can support sustainable crop production. In response to the lack of flight ready technologies capable of treating urine and gray water for water and nutrient recovery, a Suspended Aerobic Membrane Bioreactor (SAMBR) is under development between the University of South Florida and Kennedy Space Center. SAMBR is optimized for an early planetary base/partial gravity habitat and serves as a hybrid alternative to currently utilized urine treatment technologies and support closing the resource recovery loop. With its ECLSS minded form factor and modular design, SAMBR’s operation can be customized to suit the treatment objectives (i.e., nitrogen conversion) at that time. This proceeding presents preliminary research pertaining to: 1) design challenges in maximizing hydraulic throughput while minimizing mass and volume of the assembly; 2) capabilities for treating high nitrogen waste under steady and non-steady state conditions; and 3) measured performance parameters such chemical oxygen demand (COD), nitrogen conversion, nutrients, turbidity, and system throughput. Future research and development pertaining to further optimization on system safety, reliability, and expanded treatment capabilities will also be presented.

Membrane Filtration↗

Unleashing the Power of Anaerobic–Phototrophic Membrane Bioreactors for Sustainable Bioregenerative Life Support

Using the core ideals of bioregenerative life support, an anaerobic–phototrophic membrane bioreactor (APMBR) has been designed and operated at NASA’s Kennedy Space Center to treat complex wastewaters with the goal of closing water and nutrient cycles on early planetary bases. The system combined the previously operated anaerobic membrane bioreactor and the phototrophic membrane bioreactor that have been detailed in presentations at previous ICES conferences. This newly combined system is able to treat complex wastewater with completely automated controls on a small footprint. The treatment of wastes in this APMBR is as follows: (1) the waste enters the anaerobic subsystem, where solids are hydrolyzed and carbon is removed via anaerobic digestion, (2) an ultrafiltration membrane is used to separate the solids and the recovered water, (3) the effluent from the anaerobic subsystem is fed to the phototrophic subsystem on command, (4) the algae–bacteria consortium aids in nitrification in the recovered water, and (5) an ultrafiltration membrane is used to separate the algae and the final recovered water. The recovered water from the APMBR is rich in nutrients, making it a sustainable source of fertilizer for downstream hydroponic systems. This conference paper will detail the design and operation of the APMBR as a bioregenerative alternative to physical–chemical systems or bag and storage systems. In this paper, data will be presented on subsystem water quality, membrane performance, and effluent quality. Overall, the APMBR has the ability to treat wastewater using a combination of biological and filtration technologies that allow for higher removal efficiencies, low consumable use, and small footprint.

Jason A. Fischer↗