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Chen, L.

Publications and source records attributed to Chen, L..

At least 37 records · Page 2
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

Crystallization and preliminary X-ray diffraction analysis of restriction endonuclease EcoRII

Crystals of the restriction endonuclease EcoRII have been obtained by the vapor-diffusion technique in the presence of ammonium sulfate or polyethylene glycol. The best crystals were grown with ammonium sulfate as a precipitant. Crystals with dimensions of up to 0.6 x 0. 6 x 0.6 mm have been observed. The crystals diffract to about 4.0 A resolution at a cryo-temperature of 100 K using a rotating-anode X-ray source and a Rigaku R-AXIS IV imaging-plate detector. The space group has been determined to be either I23 or I2(1)3, with unit-cell parameters a = b = c = 160.3 A, alpha = beta = gamma = 90 degrees. The crystal asymmetric unit contains two protein molecules, and self-rotation function analysis shows a pseudo-twofold symmetry relating the two monomers. Attempts to improve the resolution of crystal diffraction and to search for heavy-atom derivatives are under way.

Deoxyribonucleases, Type II Site-Specific/chemistr↗
NASA NTRS1999

Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot

Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.

Non-NASA Center↗
NASA NTRS1998

Radiance Covariance and Climate Models

Spectral Empirical Orhtogonal Functions (EOFs) derived from the covariance of satellite radiance spectra may be interpreted in terms of the vertical distribution of the covariance of temperature, water vapor, and clouds. The purpose of the investigation is to demonstrate the important constraints that resolved spectral radiances can place upon climate models.

Spectral Empirical Orthogonal Functions EOFs radia↗
NASA NTRS1998

Auroral Observations from the POLAR Ultraviolet Imager (UVI)

Because of the importance of the auroral regions as a remote diagnostic of near-Earth plasma processes and magnetospheric structure, spacebased instrumentation for imaging the auroral regions have been designed and operated for the last twenty-five years. The latest generation of imagers, including those flown on the POLAR satellite, extends this quest for multispectral resolution by providing three separate imagers for the visible, ultraviolet, and X ray images of the aurora. The ability to observe extended regions allows imaging missions to significantly extend the observations available from in situ or groundbased instrumentation. The complementary nature of imaging and other observations is illustrated below using results from tile GGS Ultraviolet Imager (UVI). Details of the requisite energy and intensity analysis are also presented.

Germany, G. A.↗
NASA NTRS1998

Understanding Substorms from the Auroral Ionosphere to the Distant Plasma Sheet

The global polar UVI images have been correlated with observations from the ground, ionosphere, geomagnetic tail between 10-20 earth radii and the interplanetary space. One of the objectives of our study is to better understand the connection among many complex phenomena occurring close to Earth and those in the near--earth plasma sheet. We have examined the details of how the auroral and polar cap boundaries at different local times behave in relation to variations occurring in the solar wind, ionosphere and plasma sheet during substorms. We have also compared locations of boundaries deduced from images to electron flux "boundaries" observed by polar orbiting spacecraft. Our results indicate that the ionospheric dynamics is important and polar cap and auroral oval boundaries expand and contract in a complicated but systematic way. These variations are correlated to solar wind parameters and growth and recovery phenomena in the plasma sheet. These results can be interpreted in terms of directly driven and/or unloading substorm processes.

Parks, G. K.↗
NASA NTRS1998

Does the UVI on Polar Detect Cosmic Snowballs?

Frank and Sigwarth [1997a] claim that the dark pixels observed in dayglow images obtained by the Earth sensor of the Visible Imaging System (VIS) are due to bombardment of Earth by 20 to 40 ton cosmic snowballs. We have independently studied the same one hour of VIS data Frank and Sigwarth used and have performed detailed statistical analysis of the dark pixels. The characteristics of the dark pixels from the VIS images have been compared to those obtained from the overlapping images from the Ultraviolet Imager (UVI). We find the occurrence distributions of the dark pixels, single and multiple, from VIS and UVI are nearly identical. This result cannot be explained by a -eophysical source since the two cameras have different pixel resolutions: A search for evidence of of spacecraft "wobble" motion, whose presence would indicate that the source is external to the camera, has found that pairs of dark pixel clusters are uniformly distributed in an-le and no preference is observed in the wobble direction. Instrument artifacts as the source of the dark pixels is the most likely explanation for these results. Probability estimates for the occurrence of dark pixel clusters lead us to expect coincident events of instrumental origin to occur frequently in the two cameras. The conclusion of this study is that neither VIS nor UVI provide any scientific evidence that the origin of dark pixels is geophysical.

Parks, G. K.↗
NASA NTRS1998

Observations of Substorms from the Auroral Ionosphere to the Distant Plasma Sheet

We have been studying how substorms work by examining the global polar Ultraviolet Imager (UVI) images in correlation with observations from the ground, interplanetary space and the geomagnetic tail between 10-20 earth radii. One of the objectives of our study is to better understand the connection among many complex phenomena going on close to Earth and those in the distant plasma sheet. We have studied, for example, how the aurora[ and polar cap boundaries at different local times behave in relation to variations observed in the solar wind and plasma sheet during substorms. Preliminary results indicate that the polar cap and auroral oval boundaries expand and contract in a complicated but systematic way. These variations are correlated to solar wind parameters, and thinning and recovery phenomena in the plasma sheet. These results will be presented and interpreted in terms of directly driven and/or unloading substorm processes.

Parks, G.↗
NASA NTRS1997

Does the Ultraviolet Imager on Polar Detect Cometesimals?

The Earth's dayglow images obtained by the Ultraviolet Imager (UVI) on Polar should show darkened pixels if cometesimals bombard Earth. This darkening occurs because the UV emissions are absorbed by water between the altitude where the dayglow emissions are produced and the Polar spacecraft. We have asked: (1) Does the UVI detect darkened pixels? (2) If so, what are their characteristics? (3) What can the UVI say about the presence/absence of cometesimals? The UVI does see dark pixels, which tend to be clustered. But the calibration data obtained from the same instrument just before the instrument was launched show similarly darkened pixel clusters. After a though statistical analysis of these images, we conclude that the darkened pixels are instrumental artifacts. Therefore, the UVI does not provide evidence for the existence of the cometesimals.

Parks, G. K.↗
NASA NTRS1997

A Dayside Auroral Energy Desposition Case Study Using the Polar Ultraviolet Imager

In this letter, we report preliminary results from a study of dayside auroral energy deposition during quiet times using global auroral images acquired by the Ultraviolet Imager experiment on the Polar spacecraft. Solar wind plasma and interplanetary magnetic field measurements from the Wind spacecraft and kp values were used to characterize the state of the magnetosphere. The auroral oval was observed for a two- hour period in spring and summer during relatively quiet times (k(sub p) approx. 0 to 1). We find that, although the nightside energy deposition rate varied by an order of magnitude to as low as 1 x 10(exp 16) ergs per second (1 GW), the dayside was much less variable and remained between 4 and IO GW.

Brittnacher, M. J.↗
NASA NTRS1997

Energetics of the lattice: packing elements in crystals of four-stranded intercalated cytosine-rich DNA molecules

Condensation of single molecules from solution into crystals represents a transition between distinct energetic states. In solution, the atomic interactions within the molecule dominate. In the crystalline state, however, a set of additional interactions are formed between molecules in close contact in the lattice--these are the packing interactions. The crystal structures of d(CCCT), d(TAACCC), d(CCCAAT), and d(AACCCC) have in common a four-stranded intercalated cytosine segment, built by stacked layers of cytosine.cytosine+ (C.C+) base pairs coming from two parallel duplexes that intercalate into each other with opposite polarity. The intercalated cytosine segments in these structures are similar in their geometry, even though the sequences crystallized in different space groups. In the crystals, adenine and thymine residues of the sequences are used to build the three-dimensional crystal lattice by elaborately interacting with symmetry-related molecules. The packing elements observed provide novel insight about the copious ways in which nucleic acid molecules can interact with each other--for example, when folded in more complicated higher order structures, such as mRNA and chromatin.

Non-NASA Center↗
NASA NTRS1994

Crystal structure of a four-stranded intercalated DNA: d(C4)

The crystal structure of d(C4) solved at 2.3-A resolution reveals a four-stranded molecule composed of two interdigitated or intercalated duplexes. The duplexes are held together by hemiprotonated cytosine-cytosine base pairs and are parallel stranded, but the two duplexes point in opposite directions. The molecule has a slow right-handed twist of 12.4 degrees between covalently linked cytosine base pairs, and the base stacking distance is 3.1 A. This is in general agreement with the NMR studies. A biological role for DNA in this conformation is suggested.

Non-NASA Center↗
NASA NTRS1992

Ring current instabilities in the magnetohydrodynamic frequency range

This report summarizes recent theoretical developments in ring current plasma instabilities in the magnetohydrodynamic (MHD) frequency range but with the effect of finite Larmor radius, and discusses its relevance to satellite-based observations. Possible instabilities are the bounce resonant instabilities caused by a humped energy distribution, the drift mirror instability caused by an anisotropic pressure and the drift wave type instability caused by a combination of drift-bounce resonance and reduced Alfven frequency due to a high beta loading of the flux tube. Here, beta is proportional to plasma/magnetic pressures. Mechanisms leading to turbulence are also discussed.

Hasegawa, A.↗
NASA NTRS1989

Modern biofuel cells for waste recycling in life support systems

Innovative ways of treating urea in waste water reprocessing for long duration space exploration are being considered. Urea is very stable and therefore there are few effective ways for its decomposition. The feasibility of the use of the enzyme urease is to catalyze the hydrolysis of urea to ammonia and carbon dioxide is discussed, including a methodology, potential problems, the capabilities and advantages of such a system.

Chen, L.↗
NASA NTRS1985

The atmospheric heat budget over the western part of the Tibetan plateau during MONEX, 1979

The heat sources over the western part of the Tibetan Plateau for the period from May 19-August 31, 1979 are calculated. The use of the direct method to compute the total atmospheric heat source is examined. The values for the heat source over western, northwestern, and southwestern Tibet are presented. It is concluded that the atmospheric heat source is smaller during the summer than had been previously estimated; this difference is due to reduced drag coefficients. The northwestern and southwestern heat source values are compared, and it is observed that the northwestern heat source is independent on sensible heating and the southwestern heat source relates to the latent heat (LH). The calculated values correlate with the surface hydrology of these two regions. Time series analyses of the heat source components over the southwestern region reveal that LH is the primary modulator of the total atmospheric heat sources. The relation between the vertical heat distribution and Indian summer monsoons is studied.

Feng, Z.↗
NASA NTRS1985

Global view of the upper level outflow patterns associated with tropical cyclone intensity changes during FGGE

The characteristics of the upper tropospheric outflow patterns which occur with tropical cyclone intensification and weakening over all of the global tropical cyclone basins during the year long period of the First GARP Global Experiment (FGGE) are discussed. By intensification is meant the change in the tropical cyclone's maximum wind or central pressure, not the change of the cyclone's outer 1 to 3 deg radius mean wind which we classify as cyclone strength. All the 80 tropical cyclones which existed during the FGGE year are studied. Two-hundred mb wind fields are derived from the analysis of the European Center for Medium Range Weather Forecasting (ECMWF) which makes extensive use of upper tropospheric satellite and aircraft winds. Corresponding satellite cloud pictures from the polar orbiting U.S. Defense Meteorological Satellite Program (DMSP) and other supplementary polar and geostationary satellite data are also used.

Chen, L.↗
NASA NTRS1985

The atmospheric heat source over the Tibetan plateau May-August 1979

Estimates of the time and space variability of the atmospheric heat source over Tibet are presented for the summer of 1979. These estimates rely on new data from the People's Republic of China allowing a better assessment of the surface heat fluxes, and on new satellite data from Nimbus-7 giving the radiation balance at the top of the atmosphere. The estimates of the atmospheric heat source turned out to be considerably smaller than those provided earlier in the literature, mainly because of different assumptions of the drag coefficient. The atmospheric heat source over Tibet is mainly modulated by the release of latent heat. Over the southeastern and southwestern plateau regions the heat source appears to be in phase with the precipitation yield of the Indian summer monsoon, whereas central Tibet reveals an out-of-phase behavior. Over western Tibet there appears to be hardly any net import of moisture from outside the region, whereas the maintenance of the hydrological cycle over eastern Tibet requires moisture flux convergence from outside the region of up to 40 percent of the mean rainfall, in agreement with what is known about the surface hydrology of Tibet.

Chen, L.↗
NASA NTRS1982

Displaying Geographically-Based Domestic Statistics

Decision Information Display System (DIDS) is rapid-response information-retrieval and color-graphics display system. DIDS transforms tables of geographically-based domestic statistics (such as population or unemployment by county, energy usage by county, or air-quality figures) into high-resolution, color-coded maps on television display screen.

Quann, J.↗