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Burton, M.

Publications and source records attributed to Burton, M..

Calbindin and parvalbumin are early markers of non-mitotically regenerating hair cells in the bullfrog vestibular otolith organs

Earlier studies have demonstrated hair cell regeneration in the absence of cell proliferation, and suggested that supporting cells could phenotypically convert into hair cells following hair cell loss. Because calcium-binding proteins are involved in gene up-regulation, cell growth, and cell differentiation, we wished to determine if these proteins were up-regulated in scar formations and regenerating hair cells following gentamicin treatment. Calbindin and parvalbumin immunolabeling was examined in control or gentamicin-treated (GT) bullfrog saccular and utricular explants cultured for 3 days in amphibian culture medium or amphibian culture medium supplemented with aphidicolin, a blocker of nuclear DNA replication in eukaryotic cells. In control cultures, calbindin and parvalbumin immunolabeled the hair bundles and, less intensely, the cell bodies of mature hair cells. In GT or mitotically-blocked GT (MBGT) cultures, calbindin and parvalbumin immunolabeling was also seen in the hair bundles, cuticular plates, and cell bodies of hair cells with immature hair bundles. Thus, these antigens were useful markers for both normal and regenerating hair cells. Supporting cell immunolabeling was not seen in control cultures nor in the majority of supporting cells in GT cultures. In MBGT cultures, calbindin and parvalbumin immunolabeling was up-regulated in the cytosol of single supporting cells participating in scar formations and in supporting cells with hair cell-like characteristics. These data provide further evidence that non-mitotic hair cell regeneration in cultures can be accomplished by the conversion of supporting cells into hair cells.

Non-NASA Center↗

Heliomagnetic Latitude Dependence of Heliospheric Magnetic Field

ICE and IMP-8 magnetic field data from 1984 1988 were analyzed in a magnetic coordinate system defined by the orientation of the solar magnetic dipole. The heliomagnetic latitude dependence of the radial component of the magnetic field (Br) was then investigated in the range of magnetic latitudes from 60 above and below the heliospheric current sheet. Investigators found that Br reverses sign abruptly across the current sheet, consistent with solar magnetic field models, but inconsistent with the source surface models.

ICE↗

Heliomagnetic Latitude Dependence of the Heliospheric Magnetic Field

ICE and IMP-8 data were analyzed in a heliomagnetic coordinate system defined by the orientation of the solar magnetic dipole. Observations were compared with several solar magnetic field models. The radial component of the magnetic field was found to reverse sign abruptly at the sector boundary; this is consistent with the sheet model, but inconsistent with source surface models .

ICE↗

IR line emission from dense photodissociation regions

The results of the study on the molecular and fine structure line emission, from dense photodissociation regions, are discussed. The work is focused on the prediction of the line emission from CO and H2 molecules. It is found that, when the gas density is sufficiently high, self-shielding of the molecules can move the C(+)/CO and H/H2 transitions close to the surfaces of the molecular cloud where they can feel the full effect of heating by the ultraviolet radiation field. Collisional de-excitation of the H2 can move the lower levels towards low temperature emission producing an apparently shocked line ratio for two low-J levels, while the high-J levels retain a fluorescent value. Appreciable emission in high-J CO molecules originates in this warm molecular gas. Comparison with observation suggests that high density clumps are a common phenomena in photodissociation regions.

Burton, M.↗

High-excitation lines of molecular hydrogen: A discriminant between shock models

The results of column densities of molecular hydrogen, calculated from nineteen infrared line intensities, are discussed. They were measured at peak 1 of the outflow of the Orion molecular cloud OMC-1. The 1-0 O(7) and 0-0 S(13) lines of H2, at 3.8 microns, are mapped over the source. Their intensity ratio is found to be independent of position in the outflow. These observations are well fitted by a simple cooling-flow model of the line emitting region, but seem to be at variance with predictions of C-shocks current in the literature.

Burton, M.↗