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Bula, R. J.

Publications and source records attributed to Bula, R. J..

Experiment 8: Environmental Conditions in the ASTROCULTURE(trademark) Plant Chamber During the USML-2 Mission

Conducting plant research to assess the impact of microgravity on plant growth and development requires a plant chamber that has the capability to control other environmental parameters involved in plant growth and development. The environmental control in a space-based plant chamber must be equivalent to that available in such facilities used for terrestrial plant research. Additionally, plants are very sensitive to a number of atmospheric gaseous materials. Thus, the atmosphere of a plant chamber must be isolated from the space vehicle atmosphere, and the plant growth unit should have the capability to remove any such deleterious materials that may impact plant growth and development. The Wisconsin Center for Space Automation and Robotics (WCSAR), University of Wisconsin-Madison, has developed a totally enclosed controlled environment plant growth unit. The flight unit was used to support the ASTROCULTURE(TM) experiment conducted during the USML-2 mission. The experiment had two major objectives: 1) Provide further validation of the flight unit to control the experiment-defined environmental parameters in the plant chamber, and 2) support a plant experiment to assess the capability of potato plant material to produce tubers in microgravity. This paper describes the temperature, humidity, and carbon dioxide conditions of the plant chamber during the mission, from launch to landing. Another paper will present the plant response data.

Bula, R. J.

Plant Growth and Development in the ASTROCULTURE(trademark) Space-Based Growth Unit-Ground Based Experiments

The ASTROCULTURE(trademark) plant growth unit flown as part on the STS-63 mission in February 1995, represented the first time plants were flown in microgravity in a enclosed controlled environment plant growth facility. In addition to control of the major environmental parameters, nutrients were provided to the plants with the ZEOPONICS system developed by NASA Johnson Space Center scientists. Two plant species were included in this space experiment, dwarf wheat (Triticum aestivum) and a unique mustard called "Wisconsin Fast Plants" (Brassica rapa). Extensive post-flight analyses have been performed on the plant material and it has been concluded that plant growth and development was normal during the period the plants were in the microgravity environment of space. However, adequate plant growth and development control data were not available for direct comparisons of plant responses to the microgravity environment with those of plants grown at 1 g. Such data would allow for a more complete interpretation of the extent that microgravity affects plant growth and development.

Bula, R. J.

Capillary Movement in Substrates in Microgravity

A more complete understanding of the dynamics of capillary flow through an unsaturated porous medium would be useful for a number of space and terrestrial applications. Knowledge of capillary migration of liquids in granular beds in microgravity would significantly enhance the development and understanding of how a matrix based nutrient delivery system for the growth of plants would function in a microgravity environment. Thus, such information is of interest from the theoretical as well as practical point of view.

Bula, R. J.

Potato growth in a porous tube water and nutrient delivery system

Potato (Solanum tuberosum L.) cv. 'Norland', vegetative growth and tuber productivity grown in the porous water and nutrient delivery system (PTNDS) developed by the Wisconsin Center for Space Automation and Robotics were compared with the vegetative growth and tuber productivity of plants grown in a peat:vermiculite potting mixture (PT/VR). The plants were grown at 12, 16, and 24-h light periods, 18 degrees C constant temperature, 70% relative humidity, and 300 micromol m-2 s-1 photosynthetic photon flux. Canopy height of plants grown in the PT/VR system was taller than that of plants grown in the PTNDS system. Canopy height differences were greatest when the plants were grown under a 24-h photoperiod. Leaf and stem dry masses were similar for plants grown in the two systems under the 12-h photoperiod. Under the 24-h photoperiod, leaf and stem dry masses of plants grown in the PT/VR system were more than 3 times those of plants grown in the PTNDS system. Tuber dry masses were similar for plants grown in the two systems under the 12-h photoperiod. Under the 24 h-photoperiod, tuber dry weights of plants grown in the PT/VR system were more than twice those of plants grown in the PTNDS system. A slightly higher harvest index (ratio of tuber weight to leaf plus stem weight) was noted for the plants grown in the PTNDS than for the plants grown in the PT/VR system. Plants grown in the PTNDS system at the 24-h photoperiod matured earlier than plants grown at this photoperiod in the PT/VR system. Vegetative growth and tuber productivity of plants grown under the 16-h photoperiod generally were intermediate to those noted for plants grown under the 12 and 24-h photoperiods. These results indicate that potato plants grown in a PTNDS system may require less plant growing volume, mature in a shorter time, and likely produce more tubers per unit area compared with plants grown in the PT/VR system. These plant characteristics are a distinct advantage for a plant growing unit of a CELSS.

Non-NASA Center

Capillary movement of liquid in granular beds in microgravity

A more complete understanding of the dynamics of capillary flow through an unsaturated porous medium would be useful for the development of an effective water and nutrient delivery system for the growth of plants in space. An experiment was conducted on the Mir Space Station that used an experimental cuvette called "Capillary Test Bed" to compare fluid migration under terrestrial laboratory conditions by positioning the cuvette such that the hydrostatic force is negated and on Mir under microgravity conditions. Differences in fluid migration in the cuvette were observed with migration being slower in microgravity compared with some ground control experiments.

Mir Project

The Astroculture (tm)-1 experiment on the USML-1 mission

Permanent human presence in space will require a life support system that minimizes athe need for resupply of consumables from Earth resources. Plants that convert radiant energy to chemical energy via photosynthesis are a key component of a bioregenerative life support system. Providing the proper root environment for plants in reduced gravity is an essential aspect of the development of facilities for growing plants in a space environment. The ASTROCULTURE(TM)-1 experiment, included in the USML-1 mission, successfully demonstrated the ability of the Wisconsin Center for Space Automation and Robotics porous tube water delivery system to control water movement through a rooting matrix in a microgravity environment.

Tibbitts, Theodore

Light emitting diodes as a plant lighting source

Electroluminescence in solid materials is defined as the generation of light by the passage of an electric current through a body of solid material under an applied electric field. A specific type of electroluminescence, first noted in 1923, involves the generation of photons when electrons are passed through a p-n junction of certain solid materials (junction of a n-type semiconductor, an electron donor, and a p-type semiconductor, an electron acceptor). The development of this light emitting semiconductor technology dates back less than 30 years. During this period of time, the LED has evolved from a rare and expensive light generating device to one of the most widely used electronic components. A number of LED characteristics are of considerable importance in selecting a light source for plant lighting in a controlled environment facility. Of particular importance is the characteristic that light is generated by an LED at a rate far greater than the corresponding thermal radiation predicted by the bulk temperature of the device as defined by Plank's radiation law. This is in sharp contrast to other light sources, such as an incandescent or high intensity discharge lamp. A plant lighting system for controlled environments must provide plants with an adequate flux of photosynthetically active radiation, plus providing photons in the spectral regions that are involved in the photomorphogenic and phototropic responses that result in normal plant growth and development. Use of light sources that emit photons over a broad spectral range generally meet these two lighting requirements. Since the LED's emit over specific spectral regions, they must be carefully selected so that the levels of photsynthetically active and photomorphogenic and phototropic radiation meet these plant requirements.

Bula, R. J.

Scenarios for optimizing potato productivity in a lunar CELSS

The use of controlled ecological life support system (CELSS) in the development and growth of large-scale bases on the Moon will reduce the expense of supplying life support materials from Earth. Such systems would use plants to produce food and oxygen, remove carbon dioxide, and recycle water and minerals. In a lunar CELSS, several factors are likely to be limiting to plant productivity, including the availability of growing area, electrical power, and lamp/ballast weight for lighting systems. Several management scenarios are outlined in this discussion for the production of potatoes based on their response to irradiance, photoperiod, and carbon dioxide concentration. Management scenarios that use 12-hr photoperiods, high carbon dioxide concentrations, and movable lamp banks to alternately irradiate halves of the growing area appear to be the most efficient in terms of growing area, electrical power, and lamp weights. However, the optimal scenario will be dependent upon the relative 'costs' of each factor.

Wheeler, R. M.

Potential of derived lunar volatiles for life support

The lunar regolith contains small quantities of solar wind implanted volatile compounds that have vital, basic uses for maintaining life support systems of lunar or space settlements. Recent proposals to utilize the helium-3 isotope (He-3) derived from the lunar regolith as a fuel for fusion reactors would result in the availability of large quantities of other lunar volatile compounds. The quantities obtained would provide the annual life support replacement requirements of 1150 to 23,000 inhabitants per ton of He-3 recovered, depending on the volatile compound. Utilization of the lunar volatile compounds for life support depends on the costs, in terms of materials and energy, associated with their extraction from the lunar regolith as compared to the delivery costs of these compounds from Earth resources. Considering today's conservative estimated transportation costs ($10,000 dollars per kilogram) and regolith mining costs ($5 dollars per ton), the life support replacement requirements could be more economically supplied by recovering the lunar volatile compounds than transporting these materials from Earth resources, even before He-3 will be utilized as a fusion fuel. In addition, availability of lunar volatile compounds could have a significant cost impact on maintaining the life support systems of the space station and a Mars base.

Bula, R. J.

Importance of 'blue' photon levels for lettuce seedlings grown under red-light-emitting diodes

Light-emitting diodes (LEDs) with high-intensity output are being studied as a photosynthetic light source for plants. High-output LEDs have peak emission at approximately 660 nm concentrated in a waveband of +/- 30 nm. Lettuce (Lactuca sativa Grand Rapids') seedlings developed extended hypocotyls and elongated cotyledons when grown under these LEDs as a sole source of irradiance. This extension and elongation was prevented when the red LED radiation was supplemented with more than 15 micromoles m-2 s-1 of 400- to 500-nm photons from blue fluorescent lamps. Blue radiation effects were independent of the photon level of the red radiation.

NASA Program CELSS

Light-emitting diodes as a radiation source for plants

Development of a more effective radiation source for use in plant-growing facilities would be of significant benefit for both research and commercial crop production applications. An array of light-emitting diodes (LEDs) that produce red radiation, supplemented with a photosynthetic photon flux (PPF) of 30 micromoles s-1 m-2 in the 400- to 500-nm spectral range from blue fluorescent lamps, was used effectively as a radiation source for growing plants. Growth of lettuce (Lactuca sativa L. Grand Rapids') plants maintained under the LED irradiation system at a total PPF of 325 micromoles s-1 m-2 for 21 days was equivalent to that reported in the literature for plants grown for the same time under cool-white fluorescent and incandescent radiation sources. Characteristics of the plants, such as leaf shape, color, and texture, were not different from those found with plants grown under cool-white fluorescent lamps. Estimations of the electrical energy conversion efficiency of a LED system for plant irradiation suggest that it may be as much as twice that published for fluorescent systems.

NASA Discipline Number 61-10

Technology for subsystems of space-based plant growth facilities

Technologies for different subsystems of space-based plant growth facilities are being developed at the Wisconsin Center for Space Automation and Robotics, a NASA Center for the Commercial Development of Space. The technologies include concepts for water and nutrient delivery, for nutrient composition control, and for irradiation. Effort is being concentrated on these subsystems because available technologies cannot be effectively utilized for space applications.

Bula, R. J.

Growing plant in space

Space agencies in several countries are planning for the culture of plants in long duration space bases. The challenge of developing crop production procedures suitable for space projects will result in a new approach of problems we may meet today or in the near future in our common production systems. You may keep in mind subjects as: minimizing wastes or pollution problems, saving materials, introductions robotic helps. Discussion between scientists involved with food production for space programmes and protected horticultural cultivation may open new perspectives.

NASA Program CELSS